Moss, S;
Pretorius, E;
Ceesay, S;
Hutchins, H;
da Silva, ET;
Ndiath, MO;
Jones, RT;
Vasileva, H;
Phelan, J;
Acford-Palmer, H;
et al.
Moss, S; Pretorius, E; Ceesay, S; Hutchins, H; da Silva, ET; Ndiath, MO; Jones, RT; Vasileva, H; Phelan, J; Acford-Palmer, H; Collins, E; Rodrigues, A; Krishna, S; Clark, TG; Last, A; Campino, S
(2024)
Genomic surveillance of Anopheles mosquitoes on the Bijagós Archipelago using custom targeted amplicon sequencing identifies mutations associated with insecticide resistance.
Parasit Vectors, 17 (1).
p. 10.
ISSN 1756-3305
https://doi.org/10.1186/s13071-023-06085-5
SGUL Authors: Krishna, Sanjeev
Abstract
BACKGROUND: Insecticide resistance is reducing the efficacy of vector control interventions, consequently threatening efforts to control vector-borne diseases, including malaria. Investigating the prevalence of molecular markers of resistance is a useful tool for monitoring the spread of insecticide resistance in disease vectors. The Bijagós Archipelago (Bijagós) in Guinea-Bissau is a region of stable malaria transmission where insecticide-treated nets are the mainstay for malaria control. However, the prevalence of molecular markers of insecticide resistance in malaria vectors is not well understood. METHODS: A total of 214 Anopheles mosquitoes were analysed from 13 islands across the Bijagós. These mosquitoes were collected using CDC light traps in November 2019, during the peak malaria transmission season. High-throughput multiplex amplicon sequencing was used to investigate the prevalence of 17 different molecular markers associated with insecticide resistance in four genes: vgsc, rdl, ace1 and gste2. RESULTS: Of the 17 screened mutations, four were identified in mosquitoes from the Bijagós: vgsc L995F (12.2%), N1570Y (6.2%) and A1746S (0.7%) and rdl A269G (1.1%). This study is the first to report the L995F knock-down resistance (kdr)-west allele in Anopheles melas on the Archipelago. An additional eight non-synonymous single-nucleotide polymorphisms were identified across the four genes which have not been described previously. The prevalences of the vgsc L995F and N1570Y mutations were higher on Bubaque Island than on the other islands in this study; Bubaque is the most populous island in the archipelago, with the greatest population mobility and connection to continental Guinea-Bissau. CONCLUSIONS: This study provides the first surveillance data for genetic markers present in malaria vectors from islands across the Bijagós Archipelago. Overall prevalence of insecticide resistance mutations was found to be low. However, the identification of the vgsc L995F and N1570Y mutations associated with pyrethroid resistance warrants further monitoring. This is particularly important as the mainstay of malaria control on the islands is the use of pyrethroid insecticide-treated nets.
Item Type: |
Article
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Additional Information: |
© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
Keywords: |
Anopheles mosquitoes, Insecticide resistance, Molecular monitoring, Vector control, Animals, Anopheles, Insecticide Resistance, Insecticides, Mosquito Vectors, Pyrethrins, Genomics, Malaria, Mutation, Insecticide resistance, Vector control, Molecular monitoring, Anopheles mosquitoes, 1108 Medical Microbiology, 1117 Public Health and Health Services, Mycology & Parasitology, Tropical Medicine |
SGUL Research Institute / Research Centre: |
Academic Structure > Infection and Immunity Research Institute (INII) |
Journal or Publication Title: |
Parasit Vectors |
ISSN: |
1756-3305 |
Language: |
eng |
Dates: |
Date | Event |
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4 January 2024 | Published | 6 December 2023 | Accepted |
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Publisher License: |
Creative Commons: Attribution 4.0 |
Projects: |
|
PubMed ID: |
38178249 |
Web of Science ID: |
WOS:001136808200004 |
![](http://static.pubmed.gov/portal/portal3rc.fcgi/2745237/img/27842) |
Go to PubMed abstract |
URI: |
https://openaccess.sgul.ac.uk/id/eprint/116055 |
Publisher's version: |
https://doi.org/10.1186/s13071-023-06085-5 |
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