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Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children.

Moore, CE; Elwin, K; Phot, N; Seng, C; Mao, S; Suy, K; Kumar, V; Nader, J; Bousfield, R; Perera, S; et al. Moore, CE; Elwin, K; Phot, N; Seng, C; Mao, S; Suy, K; Kumar, V; Nader, J; Bousfield, R; Perera, S; Bailey, JW; Beeching, NJ; Day, NPJ; Parry, CM; Chalmers, RM (2016) Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children. PLoS Negl Trop Dis, 10 (7). e0004822. ISSN 1935-2735 https://doi.org/10.1371/journal.pntd.0004822
SGUL Authors: Moore, Catrin Elisabeth

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Abstract

BACKGROUND: In a prospective study, 498 single faecal samples from children aged under 16 years attending an outpatient clinic in the Angkor Hospital for Children, northwest Cambodia, were examined for Cryptosporidium oocysts and Giardia cysts using microscopy and molecular assays. METHODOLOGY/PRINCIPAL FINDINGS: Cryptosporidium oocysts were detected in 2.2% (11/498) of samples using microscopy and in 7.7% (38/498) with molecular tests. Giardia duodenalis cysts were detected in 18.9% (94/498) by microscopy and 27.7% (138/498) by molecular tests; 82% of the positive samples (by either method) were from children aged 1-10 years. Cryptosporidium hominis was the most common species of Cryptosporidium, detected in 13 (34.2%) samples, followed by Cryptosporidium meleagridis in 9 (23.7%), Cryptosporidium parvum in 8 (21.1%), Cryptosporidium canis in 5 (13.2%), and Cryptosporidium suis and Cryptosporidium ubiquitum in one sample each. Cryptosporidium hominis and C. parvum positive samples were subtyped by sequencing the GP60 gene: C. hominis IaA16R6 and C. parvum IIeA7G1 were the most abundant subtypes. Giardia duodenalis was typed using a multiplex real-time PCR targeting assemblages A and B. Assemblage B (106; 76.8% of all Giardia positive samples) was most common followed by A (12.3%) and mixed infections (5.1%). Risk factors associated with Cryptosporidium were malnutrition (AOR 9.63, 95% CI 1.67-55.46), chronic medical diagnoses (AOR 4.51, 95% CI 1.79-11.34) and the presence of birds in the household (AOR 2.99, 95% CI 1.16-7.73); specifically C. hominis (p = 0.03) and C. meleagridis (p<0.001) were associated with the presence of birds. The use of soap was protective against Giardia infection (OR 0.74, 95% CI 0.58-0.95). CONCLUSIONS/SIGNIFICANCE: This is the first report to describe the different Cryptosporidium species and subtypes and Giardia duodenalis assemblages in Cambodian children. The variety of Cryptosporidium species detected indicates both anthroponotic and zoonotic transmission in this population. Interventions to improve sanitation, increase hand washing after defecation and before preparing food and promote drinking boiled water may reduce the burden of these two parasites.

Item Type: Article
Additional Information: Copyright: © 2016 Moore et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Adolescent, Animals, Cambodia, Child, Child, Preschool, Cryptosporidiosis, Cryptosporidium, Feces, Female, Giardia, Giardiasis, Humans, Infant, Male, Prospective Studies, Feces, Animals, Humans, Cryptosporidium, Giardia, Cryptosporidiosis, Giardiasis, Prospective Studies, Adolescent, Child, Child, Preschool, Infant, Cambodia, Female, Male, 06 Biological Sciences, 11 Medical and Health Sciences, Tropical Medicine
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: PLoS Negl Trop Dis
ISSN: 1935-2735
Language: eng
Dates:
DateEvent
7 July 2016Published
13 June 2016Accepted
Publisher License: Creative Commons: Attribution 4.0
Projects:
Project IDFunderFunder ID
UNSPECIFIEDWellcome Trusthttp://dx.doi.org/10.13039/100004440
UNSPECIFIEDDepartment of Healthhttp://dx.doi.org/10.13039/501100000276
311846Seventh Framework Programmehttp://dx.doi.org/10.13039/501100004963
PubMed ID: 27387755
Web of Science ID: WOS:000381017800030
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/114482
Publisher's version: https://doi.org/10.1371/journal.pntd.0004822

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