Cooper, LP; Roberts, GA; White, JH; Luyten, YA; Bower, EKM; Morgan, RD; Roberts, RJ; Lindsay, JA; Dryden, DTF
(2017)
DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus.
Nucleic Acids Res, 45 (6).
pp. 3395-3406.
ISSN 1362-4962
https://doi.org/10.1093/nar/gkx067
SGUL Authors: Lindsay, Jodi Anne
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Abstract
Staphylococcus aureus displays a clonal population structure in which horizontal gene transfer between different lineages is extremely rare. This is due, in part, to the presence of a Type I DNA restriction–modification (RM) system given the generic name of Sau1, which maintains different patterns of methylation on specific target sequences on the genomes of different lineages. We have determined the target sequences recognized by the Sau1 Type I RM systems present in a wide range of the most prevalent S. aureus lineages and assigned the sequences recognized to particular target recognition domains within the RM enzymes. We used a range of biochemical assays on purified enzymes and single molecule real-time sequencing on genomic DNA to determine these target sequences and their patterns of methylation. Knowledge of the main target sequences for Sau1 will facilitate the synthesis of new vectors for transformation of the most prevalent lineages of this ‘untransformable’ bacterium.
Item Type: | Article | |||||||||
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Additional Information: | © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||||||
Keywords: | Developmental Biology, 05 Environmental Sciences, 06 Biological Sciences, 08 Information And Computing Sciences | |||||||||
SGUL Research Institute / Research Centre: | Academic Structure > Infection and Immunity Research Institute (INII) | |||||||||
Journal or Publication Title: | Nucleic Acids Res | |||||||||
ISSN: | 1362-4962 | |||||||||
Language: | eng | |||||||||
Publisher License: | Creative Commons: Attribution 4.0 | |||||||||
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PubMed ID: | 28180279 | |||||||||
Go to PubMed abstract | ||||||||||
URI: | https://openaccess.sgul.ac.uk/id/eprint/108590 | |||||||||
Publisher's version: | https://doi.org/10.1093/nar/gkx067 |
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