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Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay

Zhang, S; Nohturfft, A (2008) Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay. Journal of Visualized Experiments, 19 (e920). ISSN 1940-087X https://doi.org/10.3791/920
SGUL Authors: Nohturfft, Axel

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Abstract

To study the coordination of different lipid synthesis pathways during membrane biogenesis it is useful to work with an experimental system where membrane biogenesis occurs rapidly and in an inducible manner. We have found that phagocytosis of latex beads is practical for these purposes as cells rapidly synthesize membrane lipids to replenish membrane pools lost as wrapping material during particle engulfment. Here, we describe procedures for studying changes in phagocytosis-induced gene expression with a luciferase-based reporter gene approach using the Dual-Glo Luciferase Assay System from Promega.

Item Type: Article
Additional Information: Copyright © 2008 Journal of Visualized Experiments. Article made available under terms of publisher licence. Video available via publisher website.
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Journal or Publication Title: Journal of Visualized Experiments
ISSN: 1940-087X
Dates:
DateEvent
2008Published
PubMed ID: 19066535
Web of Science ID: 19066535
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URI: https://openaccess.sgul.ac.uk/id/eprint/1749
Publisher's version: https://doi.org/10.3791/920

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