Doyle, RM;
Kannambath, S;
Pittman, A;
Goliath, R;
Kumar, V;
Meintjes, G;
Milburn, J;
Netea, MG;
Harrison, TS;
Jarvis, JN;
et al.
Doyle, RM; Kannambath, S; Pittman, A; Goliath, R; Kumar, V; Meintjes, G; Milburn, J; Netea, MG; Harrison, TS; Jarvis, JN; Bicanic, T
(2024)
Ex vivo host transcriptomics during Cryptococcus neoformans, Cryptococcus gattii, and Candida albicans infection of PBMCs from South African volunteers.
The Journal of Infectious Diseases.
ISSN 0022-1899
https://doi.org/10.1093/infdis/jiae410
SGUL Authors: Pittman, Alan Michael Bicanic, Tihana
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Abstract
Cryptococcus neoformans, Cryptococcus gattii and Candida albicans are opportunistic fungal pathogens associated with infections in immunocompromised hosts. Cryptococcal meningitis (CM) is the leading fungal cause of HIV-related deaths globally, with the majority occurring in Africa. The human immune response to C. albicans infection has been studied extensively in large genomics studies whereas cryptococcal infections, despite their severity, are comparatively understudied. Here we investigated the transcriptional response of immune cells after in vitro stimulation with in vitro C. neoformans, C. gattii and C. albicans infection of peripheral blood mononuclear cells (PBMCs) collected from healthy South African volunteers. We found a lower transcriptional response to cryptococcal stimuli compared to C. albicans and unique expression signatures from all three fungal stimuli. This work provides a starting point for further studies comparing the transcriptional signature of CM in immunocompromised patients, with the goal of identifying biomarkers of disease severity and possible novel treatment targets.
Item Type: | Article | |||||||||
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Additional Information: | © The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||||||
Keywords: | 06 Biological Sciences, 11 Medical and Health Sciences, Microbiology | |||||||||
SGUL Research Institute / Research Centre: | Academic Structure > Infection and Immunity Research Institute (INII) Academic Structure > REF 2021 user group |
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Journal or Publication Title: | The Journal of Infectious Diseases | |||||||||
ISSN: | 0022-1899 | |||||||||
Language: | en | |||||||||
Dates: |
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Publisher License: | Creative Commons: Attribution 4.0 | |||||||||
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URI: | https://openaccess.sgul.ac.uk/id/eprint/116767 | |||||||||
Publisher's version: | https://doi.org/10.1093/infdis/jiae410 |
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