Rayment, N; Rhodes, G; Hudspith, B; Hughes, V; Chianini, F; Agrawal, G; Bull, TJ; Pickup, R; Sanderson, J
(2024)
Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH).
J Microbiol Methods, 224.
p. 107001.
ISSN 1872-8359
https://doi.org/10.1016/j.mimet.2024.107001
SGUL Authors: Bull, Timothy John
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Abstract
We describe the development, testing and specificity of a modified oligonucleotide probe for the specific detection of Mycobacterium avium subsp. paratuberculosis (MAP) in culture and in infected tissue using fluorescent in situ hybridisation and confocal microscopy. The detection of MAP in both animal and human tissue using our modified probe allows for a more rapid diagnosis of MAP infection compared to the more often applied detection methods of culture and PCR and has the potential for quantification of cellular abundance. This approach would enable earlier treatment intervention and therefore the potential for reduced morbidity.
Item Type: | Article | ||||||||
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Additional Information: | © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/bync/4.0/). | ||||||||
Keywords: | Crohn's disease, FISH, Johne's disease, Mycobacterium avium ssp. paratuberculosis (MAP), 0605 Microbiology, 1108 Medical Microbiology, Microbiology | ||||||||
SGUL Research Institute / Research Centre: | Academic Structure > Infection and Immunity Research Institute (INII) Academic Structure > REF 2021 user group |
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Journal or Publication Title: | J Microbiol Methods | ||||||||
ISSN: | 1872-8359 | ||||||||
Language: | eng | ||||||||
Dates: |
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PubMed ID: | 39029593 | ||||||||
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URI: | https://openaccess.sgul.ac.uk/id/eprint/116719 | ||||||||
Publisher's version: | https://doi.org/10.1016/j.mimet.2024.107001 |
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