Baruah, P; Mahony, C; Marshall, JL; Smith, CG; Monksfield, P; Irving, RI; Dumitriu, IE; Buckley, CD; Croft, AP
(2024)
Single-cell RNA sequencing analysis of vestibular schwannoma reveals functionally distinct macrophage subsets.
Br J Cancer, 130 (10).
pp. 1659-1669.
ISSN 1532-1827
https://doi.org/10.1038/s41416-024-02646-2
SGUL Authors: Dumitriu, Ingrid Elena
Abstract
BACKGROUND: Vestibular schwannomas (VSs) remain a challenge due to their anatomical location and propensity to growth. Macrophages are present in VS but their roles in VS pathogenesis remains unknown. OBJECTIVES: The objective was to assess phenotypic and functional profile of macrophages in VS with single-cell RNA sequencing (scRNAseq). METHODS: scRNAseq was carried out in three VS samples to examine characteristics of macrophages in the tumour. RT-qPCR was carried out on 10 VS samples for CD14, CD68 and CD163 and a panel of macrophage-associated molecules. RESULTS: scRNAseq revealed macrophages to be a major constituent of VS microenvironment with three distinct subclusters based on gene expression. The subclusters were also defined by expression of CD163, CD68 and IL-1β. AREG and PLAUR were expressed in the CD68+CD163+IL-1β+ subcluster, PLCG2 and NCKAP5 were expressed in CD68+CD163+IL-1β- subcluster and AUTS2 and SPP1 were expressed in the CD68+CD163-IL-1β+ subcluster. RT-qPCR showed expression of several macrophage markers in VS of which CD14, ALOX15, Interleukin-1β, INHBA and Colony Stimulating Factor-1R were found to have a high correlation with tumour volume. CONCLUSIONS: Macrophages form an important component of VS stroma. scRNAseq reveals three distinct subsets of macrophages in the VS tissue which may have differing roles in the pathogenesis of VS.
Item Type: |
Article
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Additional Information: |
© The Author(s) 2024
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
Keywords: |
1112 Oncology and Carcinogenesis, 1117 Public Health and Health Services, Oncology & Carcinogenesis |
Journal or Publication Title: |
Br J Cancer |
ISSN: |
1532-1827 |
Language: |
eng |
Dates: |
Date | Event |
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1 June 2024 | Published | 13 March 2024 | Published Online | 27 February 2024 | Accepted |
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Publisher License: |
Creative Commons: Attribution 4.0 |
Projects: |
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PubMed ID: |
38480935 |
Web of Science ID: |
WOS:001184586600002 |
![](http://static.pubmed.gov/portal/portal3rc.fcgi/2745237/img/27842) |
Go to PubMed abstract |
URI: |
https://openaccess.sgul.ac.uk/id/eprint/116447 |
Publisher's version: |
https://doi.org/10.1038/s41416-024-02646-2 |
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