Lin, G-L;
Golubchik, T;
Drysdale, S;
O'Connor, D;
Jefferies, K;
Brown, A;
de Cesare, M;
Bonsall, D;
Ansari, MA;
Aerssens, J;
et al.
Lin, G-L; Golubchik, T; Drysdale, S; O'Connor, D; Jefferies, K; Brown, A; de Cesare, M; Bonsall, D; Ansari, MA; Aerssens, J; Bont, L; Openshaw, P; Martinón-Torres, F; Bowden, R; Pollard, AJ; RESCEU Investigators
(2020)
Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants.
J Infect Dis, 222 (Suppl 7).
S666-S671.
ISSN 1537-6613
https://doi.org/10.1093/infdis/jiaa448
SGUL Authors: Drysdale, Simon Bruce
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Abstract
Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between 2 RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development. Clinical Trials Registration. NCT03627572 and NCT03756766.
| Item Type: |
Article
|
| Additional Information: |
This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Infectious Diseases following peer review. The version of record Gu-Lung Lin, Tanya Golubchik, Simon Drysdale, Daniel O’Connor, Kimberley Jefferies, Anthony Brown, Mariateresa de Cesare, David Bonsall, M Azim Ansari, Jeroen Aerssens, Louis Bont, Peter Openshaw, Federico Martinón-Torres, Rory Bowden, Andrew J Pollard, RESCEU Investigators, Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants, The Journal of Infectious Diseases, Volume 222, Issue Supplement_7, 1 November 2020, Pages S666–S671 is available online at: https://doi.org/10.1093/infdis/jiaa448 |
| Keywords: |
RNA sequencing, differential gene expression, respiratory syncytial virus, transcriptome profiling, whole-genome sequencing, Gene Expression, Gene Expression Profiling, Genome, Viral, Humans, Infant, Phylogeny, Respiratory Syncytial Virus Infections, Respiratory Syncytial Virus, Human, Sequence Analysis, Sequence Analysis, RNA, Whole Genome Sequencing, RESCEU Investigators, Humans, Respiratory Syncytial Virus, Human, Respiratory Syncytial Virus Infections, Gene Expression Profiling, Sequence Analysis, Sequence Analysis, RNA, Phylogeny, Gene Expression, Genome, Viral, Infant, Whole Genome Sequencing, respiratory syncytial virus, transcriptome profiling, RNA sequencing, whole-genome sequencing, differential gene expression, RNA sequencing, differential gene expression, respiratory syncytial virus, transcriptome profiling, whole-genome sequencing, Gene Expression, Gene Expression Profiling, Genome, Viral, Humans, Infant, Phylogeny, Respiratory Syncytial Virus Infections, Respiratory Syncytial Virus, Human, Sequence Analysis, Sequence Analysis, RNA, Whole Genome Sequencing, 06 Biological Sciences, 11 Medical and Health Sciences, Microbiology |
| SGUL Research Institute / Research Centre: |
Academic Structure > Infection and Immunity Research Institute (INII) |
| Journal or Publication Title: |
J Infect Dis |
| ISSN: |
1537-6613 |
| Language: |
eng |
| Dates: |
| Date | Event |
|---|
| 7 October 2020 | Published | | 23 July 2020 | Published Online | | 18 July 2020 | Accepted |
|
| Publisher License: |
Publisher's own licence |
| Projects: |
|
| PubMed ID: |
32702120 |
| Web of Science ID: |
WOS:000607815100016 |
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Go to PubMed abstract |
| URI: |
https://openaccess.sgul.ac.uk/id/eprint/114523 |
| Publisher's version: |
https://doi.org/10.1093/infdis/jiaa448 |
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