Yu, K;
Alzahrani, A;
Khoddami, S;
Cheng, JTJ;
Mei, Y;
Gill, A;
Luo, HD;
Haney, EF;
Hilpert, K;
Hancock, REW;
et al.
Yu, K; Alzahrani, A; Khoddami, S; Cheng, JTJ; Mei, Y; Gill, A; Luo, HD; Haney, EF; Hilpert, K; Hancock, REW; Lange, D; Kizhakkedathu, JN
(2021)
Rapid Assembly of Infection-Resistant Coatings: Screening and Identification of Antimicrobial Peptides Works in Cooperation with an Antifouling Background.
ACS Appl Mater Interfaces, 13 (31).
pp. 36784-36799.
ISSN 1944-8252
https://doi.org/10.1021/acsami.1c07515
SGUL Authors: Hilpert, Kai
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Abstract
Bacterial adhesion and the succeeding biofilm formation onto surfaces are responsible for implant- and device-associated infections. Bifunctional coatings integrating both nonfouling components and antimicrobial peptides (AMPs) are a promising approach to develop potent antibiofilm coatings. However, the current approaches and chemistry for such coatings are time-consuming and dependent on substrates and involve a multistep process. Also, the information is limited on the influence of the coating structure or its components on the antibiofilm activity of such AMP-based coatings. Here, we report a new strategy to rapidly assemble a stable, potent, and substrate-independent AMP-based antibiofilm coating in a nonfouling background. The coating structure allowed for the screening of AMPs in a relevant nonfouling background to identify optimal peptide combinations that work in cooperation to generate potent antibiofilm activity. The structure of the coating was changed by altering the organization of the hydrophilic polymer chains within the coatings. The coatings were thoroughly characterized using various surface analytical techniques and correlated with the efficiency to prevent biofilm formation against diverse bacteria. The coating method that allowed the conjugation of AMPs without altering the steric protection ability of hydrophilic polymer structure results in a bifunctional surface coating with excellent antibiofilm activity. In contrast, the conjugation of AMPs directly to the hydrophilic polymer chains resulted in a surface with poor antibiofilm activity and increased adhesion of bacteria. Using this coating approach, we further established a new screening method and identified a set of potent surface-tethered AMPs with high activity. The success of this new peptide screening and coating method is demonstrated using a clinically relevant mouse infection model to prevent catheter-associated urinary tract infection (CAUTI).
Item Type: |
Article
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Additional Information: |
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials and Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsami.1c07515 |
Keywords: |
antibiofilm coating, antimicrobial peptides, bifunctional coating, implant-associated infection, screening method, substrate-independent coating, antibiofilm coating, antimicrobial peptides, bifunctional coating, implant-associated infection, screening method, substrate-independent coating, 0904 Chemical Engineering, 0303 Macromolecular and Materials Chemistry, 0306 Physical Chemistry (incl. Structural), Nanoscience & Nanotechnology |
SGUL Research Institute / Research Centre: |
Academic Structure > Infection and Immunity Research Institute (INII) |
Journal or Publication Title: |
ACS Appl Mater Interfaces |
ISSN: |
1944-8252 |
Language: |
eng |
Dates: |
Date | Event |
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11 August 2021 | Published | 30 July 2021 | Published Online | 1 July 2021 | Accepted |
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Publisher License: |
Publisher's own licence |
Projects: |
|
PubMed ID: |
34328312 |
![](http://static.pubmed.gov/portal/portal3rc.fcgi/2745237/img/27842) |
Go to PubMed abstract |
URI: |
https://openaccess.sgul.ac.uk/id/eprint/113526 |
Publisher's version: |
https://doi.org/10.1021/acsami.1c07515 |
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