Tien, N-Q-D; Yang, M-S; Jang, Y-S; Kwon, T-H; Reljic, R; Kim, M-Y
(2021)
Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants.
Front Pharmacol, 12.
p. 653064.
ISSN 1663-9812
https://doi.org/10.3389/fphar.2021.653064
SGUL Authors: Reljic, Rajko
Abstract
Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of the core neutralising epitope (COE) of PEDV conjugated to polymeric immunoglobulin G scaffold (PIGS) in glycoengineered Nicotiana benthamiana plants. The biological activity of COE-PIGS was demonstrated by binding to C1q component of the complement system, as well as the surface of antigen-presenting cells (APCs) in vitro. The recombinant COE-PIGS induced humoral and cellular immune responses specific for PEDV after both systemic and mucosal vaccination. Altogether, the data indicated that PEDV antigen fusion to poly-Fc could be a promising vaccine platform against respiratory PEDV infection.
Item Type: |
Article
|
Additional Information: |
Copyright © 2021 Tien, Yang, Jang, Kwon, Reljic and Kim. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Keywords: |
PEDV, immunity, mucosal, plant, vaccine, vaccine, PEDV, mucosal, immunity, plant, 1115 Pharmacology and Pharmaceutical Sciences |
SGUL Research Institute / Research Centre: |
Academic Structure > Infection and Immunity Research Institute (INII) |
Journal or Publication Title: |
Front Pharmacol |
ISSN: |
1663-9812 |
Language: |
eng |
Dates: |
Date | Event |
---|
30 April 2021 | Published | 16 March 2021 | Accepted |
|
Publisher License: |
Creative Commons: Attribution 4.0 |
Projects: |
Project ID | Funder | Funder ID |
---|
714001-07 | Korean Ministry of Agriculture, Food and Rural Affairs | UNSPECIFIED | NRF-2020K1A4A7A02095058 | National Research Foundation of Korea | http://dx.doi.org/10.13039/501100003725 | MC-PC-18069 | Korea-UK partnership grant | UNSPECIFIED |
|
PubMed ID: |
33995068 |
Web of Science ID: |
WOS:000650202900001 |
![](http://static.pubmed.gov/portal/portal3rc.fcgi/2745237/img/27842) |
Go to PubMed abstract |
URI: |
https://openaccess.sgul.ac.uk/id/eprint/113288 |
Publisher's version: |
https://doi.org/10.3389/fphar.2021.653064 |
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