Brown, L;
Byrne, RL;
Fraser, A;
Owen, SI;
Cubas-Atienzar, AI;
Williams, CT;
Kay, GA;
Cuevas, LE;
Fitchett, JRA;
Fletcher, T;
et al.
Brown, L; Byrne, RL; Fraser, A; Owen, SI; Cubas-Atienzar, AI; Williams, CT; Kay, GA; Cuevas, LE; Fitchett, JRA; Fletcher, T; Garrod, G; Kontogianni, K; Krishna, S; Menzies, S; Planche, T; Sainter, C; Staines, HM; Turtle, L; Adams, ER
(2021)
Self-sampling of capillary blood for SARS-CoV-2 serology.
Scientific Reports, 11 (1).
p. 7754.
ISSN 2045-2322
https://doi.org/10.1038/s41598-021-86008-5
SGUL Authors: Staines, Henry Michael
Abstract
Serological testing is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission and immune response. Large-scale testing is limited by the need for in-person blood collection by staff trained in venepuncture, and the limited sensitivity of lateral flow tests. Capillary blood self-sampling and postage to laboratories for analysis could provide a reliable alternative. Two-hundred and nine matched venous and capillary blood samples were obtained from thirty nine participants and analysed using a COVID-19 IgG ELISA to detect antibodies against SARS-CoV-2. Thirty eight out of thirty nine participants were able to self-collect an adequate sample of capillary blood (≥ 50 µl). Using plasma from venous blood collected in lithium heparin as the reference standard, matched capillary blood samples, collected in lithium heparin-treated tubes and on filter paper as dried blood spots, achieved a Cohen’s kappa coefficient of > 0.88 (near-perfect agreement, 95% CI 0.738–1.000). Storage of capillary blood at room temperature for up to 7 days post sampling did not affect concordance. Our results indicate that capillary blood self-sampling is a reliable and feasible alternative to venepuncture for serological assessment in COVID-19.
Item Type: |
Article
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Additional Information: |
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
© The Author(s) 2021 |
SGUL Research Institute / Research Centre: |
Academic Structure > Infection and Immunity Research Institute (INII) |
Journal or Publication Title: |
Scientific Reports |
ISSN: |
2045-2322 |
Language: |
en |
Dates: |
Date | Event |
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December 2021 | Published | 8 April 2021 | Published Online | 18 January 2021 | Accepted |
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Publisher License: |
Creative Commons: Attribution 4.0 |
Projects: |
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URI: |
https://openaccess.sgul.ac.uk/id/eprint/113149 |
Publisher's version: |
https://doi.org/10.1038/s41598-021-86008-5 |
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