Dertinger, SD; Bhalli, JA; Roberts, DJ; Stankowski, LF; Gollapudi, BB; Lovell, DP; Recio, L; Kimoto, T; Miura, D; Heflich, RH
(2021)
Recommendations for conducting the rodent erythrocyte Pig-a assay: A report from the HESI GTTC Pig-a Workgroup.
Environ Mol Mutagen, 62 (3).
pp. 227-237.
ISSN 1098-2280
https://doi.org/10.1002/em.22427
SGUL Authors: Lovell, David
Abstract
The rodent Pig-a assay is a flow cytometric, phenotype-based method used to measure in vivo somatic cell mutation. An Organization for Economic Co-operation and Development (OECD) test guideline is currently being developed to support routine use of the assay for regulatory purposes (OECD project number 4.93). This article provides advice on best practices for designing and conducting rodent Pig-a studies in support of evaluating test substance safety, with a focus on the rat model. Various aspects of assay conduct, including laboratory proficiency, minimum number of animals per dose group, preferred treatment and blood sampling schedule, and statistical analysis are described.
Item Type: |
Article
|
Additional Information: |
© 2021 The Authors. Environmental and Molecular Mutagenesis published by Wiley Periodicals LLC on behalf of Environmental Mutagen Society.
This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: |
Pig-a gene, flow cytometry, genotoxicity, mutagen, mutation assay, flow cytometry, genotoxicity, mutagen, mutation assay, Pig‐, a gene, Toxicology, 05 Environmental Sciences, 06 Biological Sciences, 11 Medical and Health Sciences |
SGUL Research Institute / Research Centre: |
Academic Structure > Population Health Research Institute (INPH) |
Journal or Publication Title: |
Environ Mol Mutagen |
ISSN: |
1098-2280 |
Language: |
eng |
Dates: |
Date | Event |
---|
11 March 2021 | Published | 2 March 2021 | Published Online | 17 February 2021 | Accepted |
|
Publisher License: |
Creative Commons: Attribution 4.0 |
Projects: |
Project ID | Funder | Funder ID |
---|
R44 ES028163 | NIEHS NIH HHS | UNSPECIFIED | R44-ES028163 | National Institutes of Health, National Institute of Environmental Health Sciences | UNSPECIFIED |
|
PubMed ID: |
33608913 |
Web of Science ID: |
WOS:000624016200001 |
![](http://static.pubmed.gov/portal/portal3rc.fcgi/2745237/img/27842) |
Go to PubMed abstract |
URI: |
https://openaccess.sgul.ac.uk/id/eprint/113148 |
Publisher's version: |
https://doi.org/10.1002/em.22427 |
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