Raimondi, C; Fantin, A; Lampropoulou, A; Denti, L; Chikh, A; Ruhrberg, C
(2014)
Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells.
J Exp Med, 211 (6).
pp. 1167-1183.
ISSN 1540-9538
https://doi.org/10.1084/jem.20132330
SGUL Authors: Chikh, Anissa
Abstract
To enable new blood vessel growth, endothelial cells (ECs) express neuropilin 1 (NRP1), and NRP1 associates with the receptor tyrosine kinase VEGFR2 after binding the vascular endothelial growth factor A (VEGF) to enhance arteriogenesis. We report that NRP1 contributes to angiogenesis through a novel mechanism. In human and mouse ECs, the integrin ligand fibronectin (FN) stimulated actin remodeling and phosphorylation of the focal adhesion component paxillin (PXN) in a VEGF/VEGFR2-independent but NRP1-dependent manner. NRP1 formed a complex with ABL1 that was responsible for FN-dependent PXN activation and actin remodeling. This complex promoted EC motility in vitro and during angiogenesis on FN substrates in vivo. Accordingly, both physiological and pathological angiogenesis in the retina were inhibited by treatment with Imatinib, a small molecule inhibitor of ABL1 which is widely used to prevent the proliferation of tumor cells that express BCR-ABL fusion proteins. The finding that NRP1 regulates angiogenesis in a VEGF- and VEGFR2-independent fashion via ABL1 suggests that ABL1 inhibition provides a novel opportunity for anti-angiogenic therapy to complement VEGF or VEGFR2 blockade in eye disease or solid tumor growth.
Item Type: |
Article
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Additional Information: |
© 2014 Raimondi et al.
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
Keywords: |
Animals, Benzamides, Cell Adhesion, Cell Movement, Cells, Cultured, Endothelial Cells, Enzyme Activation, Fibronectins, Humans, Imatinib Mesylate, Immunoblotting, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Microscopy, Confocal, Neovascularization, Physiologic, Neuropilin-1, Paxillin, Phosphorylation, Piperazines, Protein Kinase Inhibitors, Proto-Oncogene Proteins c-abl, Pyrimidines, RNA Interference, Retinal Neovascularization, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor Receptor-2, Cells, Cultured, Endothelial Cells, Animals, Mice, Inbred C57BL, Mice, Transgenic, Mice, Knockout, Humans, Mice, Retinal Neovascularization, Benzamides, Piperazines, Pyrimidines, Vascular Endothelial Growth Factor Receptor-2, Proto-Oncogene Proteins c-abl, Vascular Endothelial Growth Factor A, Fibronectins, Neuropilin-1, Protein Kinase Inhibitors, Microscopy, Confocal, Immunoblotting, Cell Adhesion, Cell Movement, RNA Interference, Enzyme Activation, Phosphorylation, Neovascularization, Physiologic, Paxillin, Imatinib Mesylate, 11 Medical and Health Sciences, Immunology |
SGUL Research Institute / Research Centre: |
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) |
Journal or Publication Title: |
J Exp Med |
ISSN: |
1540-9538 |
Language: |
eng |
Dates: |
Date | Event |
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2 June 2014 | Published | 14 April 2014 | Accepted |
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Publisher License: |
Creative Commons: Attribution-Noncommercial-Share Alike 3.0 |
Projects: |
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PubMed ID: |
24863063 |
Web of Science ID: |
WOS:000337364500013 |
![](http://static.pubmed.gov/portal/portal3rc.fcgi/2745237/img/27842) |
Go to PubMed abstract |
URI: |
https://openaccess.sgul.ac.uk/id/eprint/111983 |
Publisher's version: |
https://doi.org/10.1084/jem.20132330 |
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