Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection.

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Mateos, J; Estévez, O; González-Fernández, Á; Anibarro, L; Pallarés, Á; Reljic, R; Mussá, T; Gomes-Maueia, C; Nguilichane, A; Gallardo, JM; et al. Mateos, J; Estévez, O; González-Fernández, Á; Anibarro, L; Pallarés, Á; Reljic, R; Mussá, T; Gomes-Maueia, C; Nguilichane, A; Gallardo, JM; Medina, I; Carrera, M (2020) Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection. Sci Rep, 10 (1). p. 3844. ISSN 2045-2322 https://doi.org/10.1038/s41598-020-60753-5
SGUL Authors: Reljic, Rajko

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Abstract

Tuberculosis (TB) is the most lethal infection among infectious diseases. The specific aim of this study was to establish panels of serum protein biomarkers representative of active TB patients and their household contacts who were either infected (LTBI) or uninfected (EMI-TB Discovery Cohort, Pontevedra Region, Spain). A TMT (Tamdem mass tags) 10plex-based quantitative proteomics study was performed in quintuplicate containing a total of 15 individual serum samples per group. Peptides were analyzed in an LC-Orbitrap Elite platform, and raw data were processed using Proteome Discoverer 2.1. A total of 418 proteins were quantified. The specific protein signature of active TB patients was characterized by an accumulation of proteins related to complement activation, inflammation and modulation of immune response and also by a decrease of a small subset of proteins, including apolipoprotein A and serotransferrin, indicating the importance of lipid transport and iron assimilation in the progression of the disease. This signature was verified by the targeted measurement of selected candidates in a second cohort (EMI-TB Verification Cohort, Maputo Region, Mozambique) by ELISA and nephelometry techniques. These findings will aid our understanding of the complex metabolic processes associated with TB progression from LTBI to active disease.

Item Type:

Article

Additional Information:

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2020

SGUL Research Institute / Research Centre:

Academic Structure > Infection and Immunity Research Institute (INII)

Journal or Publication Title:

Sci Rep

ISSN:

2045-2322

Language:

eng

Dates: