Diogo, GR; Hart, P; Copland, A; Kim, M-Y; Tran, AC; Poerio, N; Singh, M; Paul, MJ; Fraziano, M; Reljic, R
(2019)
Immunization With Mycobacterium tuberculosis Antigens Encapsulated in Phosphatidylserine Liposomes Improves Protection Afforded by BCG.
Front Immunol, 10.
p. 1349.
ISSN 1664-3224
https://doi.org/10.3389/fimmu.2019.01349
SGUL Authors: Paul, Mathew John Reljic, Rajko
Abstract
Liposomes have been long considered as a vaccine delivery system but this technology remains to be fully utilized. Here, we describe a novel liposome-based subunit vaccine formulation for tuberculosis (TB) based on phosphatidylserine encapsulating two prominent TB antigens, Ag85B, and ESAT-6. We show that the resulting liposomes (Lipo-AE) are stable upon storage and can be readily taken up by antigen presenting cells and that their antigenic cargo is delivered and processed within endosomal cell compartments. The Lipo-AE vaccine formulation combined with the PolyIC adjuvant induced a mixed Th1/Th17-Th2 immune response to Ag85B but only a weak response to ESAT-6. An immunization regimen based on systemic delivery followed by mucosal boost with Lipo-AE resulted in the accumulation of resident memory T cells in the lungs. Most importantly though, when Lipo-AE vaccine candidate was administered to BCG-immunized mice subsequently challenged with low dose aerosol Mycobacterium tuberculosis, we observed a significant reduction of the bacterial load in the lungs and spleen compared to BCG alone. We therefore conclude that the immunization with mycobacterial antigens delivered by phosphatidylserine based liposomes in combination with Poly:IC adjuvant may represent a novel BCG boosting vaccination strategy.
Item Type: |
Article
|
Additional Information: |
Copyright © 2019 Diogo, Hart, Copland, Kim, Tran, Poerio, Singh, Paul, Fraziano and Reljic. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Keywords: |
BCG, immunity, liposomes, tuberculosis, vaccine, liposomes, tuberculosis, vaccine, BCG, immunity, liposomes, tuberculosis, vaccine, BCG, immunity |
SGUL Research Institute / Research Centre: |
Academic Structure > Infection and Immunity Research Institute (INII) |
Journal or Publication Title: |
Front Immunol |
ISSN: |
1664-3224 |
Language: |
eng |
Dates: |
Date | Event |
---|
12 June 2019 | Published | 28 May 2019 | Accepted |
|
Publisher License: |
Creative Commons: Attribution 4.0 |
Projects: |
Project ID | Funder | Funder ID |
---|
643558 | European Community H2020 | UNSPECIFIED |
|
PubMed ID: |
31293568 |
Web of Science ID: |
WOS:000471194800001 |
![](http://static.pubmed.gov/portal/portal3rc.fcgi/2745237/img/27842) |
Go to PubMed abstract |
URI: |
https://openaccess.sgul.ac.uk/id/eprint/111010 |
Publisher's version: |
https://doi.org/10.3389/fimmu.2019.01349 |
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