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Novel Method for the Purification of House Dust Mite Allergen Der p 1 and Its Use in Structure-Based Chemical Design of Novel Inhibitors.

Zhang, J; Robinson, C (2019) Novel Method for the Purification of House Dust Mite Allergen Der p 1 and Its Use in Structure-Based Chemical Design of Novel Inhibitors. Methods Mol Biol, 2020. pp. 185-205. ISSN 1940-6029 https://doi.org/10.1007/978-1-4939-9591-2_14
SGUL Authors: Robinson, Clive

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Abstract

House dust mites are globally significant triggers of allergic disease. Notable among their extensive repertoire of allergens are the Group 1 cysteine peptidase allergens which function as digestive enzymes in house dust mites. Compelling evidence suggests that the proteolytic activity of these molecules plays a key role in the development and maintenance of allergic diseases through the activation of innate immune mechanisms which exploit genetic predispositions to allergy. Growing interest in this area creates a requirement for high-quality purified protein, whether natural or recombinantly expressed. It has also identified these allergens as therapeutic targets for a novel approach to allergy treatment through modulation of innate immune responses. The purpose of this chapter is to describe a new method for the purification of Der p 1 and use of the protein produced in a screening assay designed for the discovery of novel inhibitors of Group 1 house dust mite allergens.

Item Type: Article
Additional Information: This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-9591-2_14
Keywords: Allergen delivery inhibitors, Der p 1 purification, House dust mite, Innate immunity, Protease assay, Protein purification, Structure-based drug design, 0601 Biochemistry And Cell Biology, Developmental Biology
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: Methods Mol Biol
ISSN: 1940-6029
Language: eng
Dates:
DateEvent
9 June 2019Published
Publisher License: Publisher's own licence
PubMed ID: 31177501
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/110929
Publisher's version: https://doi.org/10.1007/978-1-4939-9591-2_14

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