Long, JS;
Idoko-Akoh, A;
Mistry, B;
Goldhill, D;
Staller, E;
Schreyer, J;
Ross, C;
Goodbourn, S;
Shelton, H;
Skinner, MA;
et al.
Long, JS; Idoko-Akoh, A; Mistry, B; Goldhill, D; Staller, E; Schreyer, J; Ross, C; Goodbourn, S; Shelton, H; Skinner, MA; Sang, H; McGrew, MJ; Barclay, W
(2019)
Species specific differences in use of ANP32 proteins by influenza A virus.
Elife, 8.
e45066.
ISSN 2050-084X
https://doi.org/10.7554/eLife.45066
SGUL Authors: Goodbourn, Stephen Edward Ross, Craig Stephen
Abstract
Influenza A viruses (IAV) are subject to species barriers that prevent frequent zoonotic transmission and pandemics. One of these barriers is the poor activity of avian IAV polymerases in human cells. Differences between avian and mammalian ANP32 proteins underlie this host range barrier. Human ANP32A and ANP32B homologues both support function of human-adapted influenza polymerase but do not support efficient activity of avian IAV polymerase which requires avian ANP32A. We show here that the gene currently designated as avian ANP32B is evolutionarily distinct from mammalian ANP32B, and that chicken ANP32B does not support IAV polymerase activity even of human-adapted viruses. Consequently, IAV relies solely on chicken ANP32A to support its replication in chicken cells. Amino acids 129I and 130N, accounted for the inactivity of chicken ANP32B. Transfer of these residues to chicken ANP32A abolished support of IAV polymerase. Understanding ANP32 function will help develop antiviral strategies and aid the design of influenza virus resilient genome edited chickens.
Item Type: |
Article
|
Additional Information: |
© 2019, Long et al.
This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited. |
Keywords: |
ANP32A, ANP32B, Influenza, chicken, gene editing, human, infectious disease, microbiology, polymerase, virus |
SGUL Research Institute / Research Centre: |
Academic Structure > Infection and Immunity Research Institute (INII) |
Journal or Publication Title: |
Elife |
ISSN: |
2050-084X |
Language: |
eng |
Dates: |
Date | Event |
---|
4 June 2019 | Published | 29 April 2019 | Accepted |
|
Publisher License: |
Creative Commons: Attribution 4.0 |
Projects: |
|
PubMed ID: |
31159925 |
![](http://static.pubmed.gov/portal/portal3rc.fcgi/2745237/img/27842) |
Go to PubMed abstract |
URI: |
https://openaccess.sgul.ac.uk/id/eprint/110840 |
Publisher's version: |
https://doi.org/10.7554/eLife.45066 |
Statistics
Item downloaded times since 18 Jun 2019.
Actions (login required)
![Edit Item](/style/images/action_view.png) |
Edit Item |