Jonas, KC; Hanyaloglu, AC
(2019)
Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy.
In:
G Protein-Coupled Receptor Signaling: Methods and Protocols.
Methods in Molecular Biology
(1947).
(ed Tiberi, M.)
Humana Press (Springer Imprint), pp. 337-348.
DOI: https://doi.org/10.1007/978-1-4939-9121-1
SGUL Authors: Jonas, Kim Carol
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Abstract
Super-resolution imaging has provided unprecedented insight in the molecular complexities of fundamental cell biological questions. For G protein-coupled receptors (GPCRs), its application to the study of receptor homomers and heteromers have unveiled the diversity of complexes these GPCRs can form at the plasma membrane at a structural and functional level. Here, we describe our methodological approach of photoactivated localization microscopy with photoactivatable dyes (PD-PALM) to visualize and quantify the spatial assembly of GPCR heteromers at the plasma membrane.
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