Carr, DJ; David, AL; Aitken, RP; Milne, JS; Borowicz, PP; Wallace, JM; Redmer, DA
(2016)
Placental vascularity and markers of angiogenesis in relation to prenatal growth status in overnourished adolescent ewes.
Placenta, 46.
pp. 79-86.
ISSN 1532-3102
https://doi.org/10.1016/j.placenta.2016.08.076
SGUL Authors: Carr, David John
Abstract
INTRODUCTION: Placental vascularity may be important in the development of fetal growth restriction (FGR). The overnourished adolescent ewe is a robust model of the condition, with ∼50% of offspring demonstrating FGR (birthweight >2 standard deviations below optimally-fed control mean). We studied whether placental vascularity, angiogenesis and glucose transport reflect FGR severity. METHODS: Singleton pregnancies were established in adolescent ewes either overnourished to putatively restrict fetoplacental growth (n = 27) or control-fed (n = 12). At 131d (term = 145d) pregnancies were interrupted and fetuses classified as FGR (n = 17, <4222 g, -2SD below control-fed mean) or non-FGR (n = 10). Placentome capillary area density (CAD), number density (CND), surface density (CSD), and area per capillary (APC) in the fetal cotyledon (COT) and maternal caruncle (CAR) were analysed using immunostaining. COT/CAR mRNA expression of angiogenic ligands/receptors and glucose transporters were measured by qRT-PCR. RESULTS: Fetal weight was reduced in FGR vs. Non-FGR/Control groups. Total placentome weight was Control > Non-FGR > FGR and fetal:placental weight ratios were higher in overnourished versus Control groups. COT vascular indices were Non-FGR > FGR > Control. COT-CAD, CSD and APC were significantly greater in Non-FGR overnourished versus Control and intermediate in FGR groups. CAR vascularity did not differ. CAR-VEGFA/FLT1/KDR/ANGPT1/ANGPT2/SLC2A1/SLC2A3 mRNA was lower and COT-ANGPT2 higher in overnourished versus Control groups. DISCUSSION: Relative to control-intake pregnancy, overnourished pregnancies are characterised by higher COT vascularity, potentially a compensatory response to reduced nutrient supply, reflected by higher fetal:placental weight ratios. Compared with overnourished pregnancies where fetal growth is relatively preserved, overnourished pregnancies culminating in marked FGR have less placental vascularity, suggesting incomplete adaptation to the prenatal insult.
Item Type: |
Article
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Additional Information: |
© 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: |
Angiogenic factors, Angiopoietin, Fetal growth restriction, Placental vascularity, Sheep, Animals, Biomarkers, Disease Models, Animal, Female, Fetal Growth Retardation, Neovascularization, Physiologic, Placenta, Pregnancy, Pregnancy Outcome, Sheep, Ultrasonography, Prenatal, Placental vascularity, Fetal growth restriction, Angiogenic factors, Sheep, Angiopoietin, Obstetrics & Reproductive Medicine, 1103 Clinical Sciences, 1114 Paediatrics And Reproductive Medicine |
SGUL Research Institute / Research Centre: |
Academic Structure > Institute of Medical & Biomedical Education (IMBE) |
Journal or Publication Title: |
Placenta |
ISSN: |
1532-3102 |
Language: |
eng |
Dates: |
Date | Event |
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October 2016 | Published | 19 August 2016 | Published Online | 16 August 2016 | Accepted |
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Publisher License: |
Creative Commons: Attribution 4.0 |
Projects: |
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PubMed ID: |
27697225 |
Web of Science ID: |
WOS:000386409400011 |
![](http://static.pubmed.gov/portal/portal3rc.fcgi/2745237/img/27842) |
Go to PubMed abstract |
URI: |
https://openaccess.sgul.ac.uk/id/eprint/109867 |
Publisher's version: |
https://doi.org/10.1016/j.placenta.2016.08.076 |
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