Kim, MY;
Copland, A;
Nayak, K;
Chandele, A;
Ahmed, MS;
Zhang, Q;
Diogo, GR;
Paul, MJ;
Hofmann, S;
Yang, M-S;
et al.
Kim, MY; Copland, A; Nayak, K; Chandele, A; Ahmed, MS; Zhang, Q; Diogo, GR; Paul, MJ; Hofmann, S; Yang, M-S; Jang, Y-S; Ma, JK-C; Reljic, R
(2018)
Plant-expressed Fc-fusion protein tetravalent dengue vaccine with inherent adjuvant properties.
Plant Biotechnol J, 16 (7).
pp. 1283-1294.
ISSN 1467-7652
https://doi.org/10.1111/pbi.12869
SGUL Authors: Hofmann, Sven
Abstract
Dengue is a major global disease requiring improved treatment and prevention strategies. The recently licensed Sanofi-Pasteur Denvaxia vaccine does not protect children under the age of nine and additional vaccine strategies are thus needed to halt this expanding global epidemic. Here, we employed a molecular engineering approach and plant-expression to produce a humanised and highly immunogenic Poly-Immunoglobulin G Scaffold (PIGS) fused to the consensus dengue envelope protein III domain (cEDIII). The immunogenicity of this IgG Fc receptor targeted vaccine candidate was demonstrated in transgenic mice expressing human FcγRI/CD64, by induction of neutralising antibodies and evidence of cell-mediated immunity. Furthermore, these molecules were able to prime immune cells from human adenoid/tonsillar tissue ex vivo as evidenced by antigen-specific CD4+ and CD8+ T cell proliferation, IFN-γ and antibody production. The purified polymeric fraction of dengue PIGS (D-PIGS) induced stronger immune activation than the monomeric form, suggesting a more efficient interaction with the low affinity Fcγ receptors on antigen-presenting cells. These results show that the plant-expressed D-PIGS have the potential for translation towards a safe and easily scalable single antigen based tetravalent dengue vaccine. This article is protected by copyright. All rights reserved.
Item Type: |
Article
|
Additional Information: |
© 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: |
Dengue, Fc-fusion proteins, IgG, Vaccine, human, neutralising antibodies, Biotechnology, 10 Technology, 06 Biological Sciences, 11 Medical And Health Sciences |
Journal or Publication Title: |
Plant Biotechnol J |
ISSN: |
1467-7652 |
Language: |
eng |
Dates: |
Date | Event |
---|
18 June 2018 | Published | 1 February 2018 | Published Online | 9 December 2017 | Published Online | 3 December 2017 | Accepted |
|
Publisher License: |
Creative Commons: Attribution 4.0 |
Projects: |
Project ID | Funder | Funder ID |
---|
2013R1A6A3A03022769 | Korean Ministry of Science, ICT and Future Planning | UNSPECIFIED | NRF-2014K1B1A10738 | Korean Ministry of Science, ICT and Future Planning | UNSPECIFIED |
|
PubMed ID: |
29223138 |
![](http://static.pubmed.gov/portal/portal3rc.fcgi/2745237/img/27842) |
Go to PubMed abstract |
URI: |
https://openaccess.sgul.ac.uk/id/eprint/109586 |
Publisher's version: |
https://doi.org/10.1111/pbi.12869 |
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