Spencer, NG; Lovell, DP; Elderfield, K; Austen, BM; Howe, FA
(2017)
Can MRI T1 be used to detect early changes in 5xFAD Alzheimer’s mouse brain?
MAGMA Magnetic Resonance Materials in Physics, Biology and Medicine, 30 (2).
pp. 153-163.
https://doi.org/10.1007/s10334-016-0593-9
SGUL Authors: Howe, Franklyn Arron
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Abstract
In the present study, we have tested whether MRI T1 relaxation time is a sensitive marker to detect early stages of amyloidosis and gliosis in the young 5xFAD transgenic mouse, a well-established animal model for Alzheimer's disease.5xFAD and wild-type mice were imaged in a 4.7 T Varian horizontal bore MRI system to generate T1 quantitative maps using the spin-echo multi-slice sequence. Following immunostaining for glial fibrillary acidic protein, Iba-1, and amyloid-β, T1 and area fraction of staining were quantified in the posterior parietal and primary somatosensory cortex and corpus callosum.In comparison with age-matched wild-type mice, we observed first signs of amyloidosis in 2.5-month-old 5xFAD mice, and development of gliosis in 5-month-old 5xFAD mice. In contrast, MRI T1 relaxation times of young, i.e., 2.5- and 5-month-old, 5xFAD mice were not significantly different to those of age-matched wild-type controls. Furthermore, although disease progression was detectable by increased amyloid-β load in the brain of 5-month-old 5xFAD mice compared with 2.5-month-old 5xFAD mice, MRI T1 relaxation time did not change.In summary, our data suggest that MRI T1 relaxation time is neither a sensitive measure of disease onset nor progression at early stages in the 5xFAD mouse transgenic mouse model.
Item Type: | Article | ||||||
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Additional Information: | © The Author(s) 2016. Open Access: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | ||||||
Keywords: | Nuclear Medicine & Medical Imaging, MD Multidisciplinary | ||||||
SGUL Research Institute / Research Centre: | Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Neuroscience (INCCNS) |
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Journal or Publication Title: | MAGMA Magnetic Resonance Materials in Physics, Biology and Medicine | ||||||
Publisher License: | Creative Commons: Attribution 4.0 | ||||||
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URI: | https://openaccess.sgul.ac.uk/id/eprint/108339 | ||||||
Publisher's version: | https://doi.org/10.1007/s10334-016-0593-9 |
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