Meireles, P; Sales-Dias, J; Andrade, CM; Mello-Vieira, J; Mancio-Silva, L; Simas, JP; Staines, HM; Prudêncio, M
(2017)
GLUT1-mediated glucose uptake plays a crucial role during Plasmodium hepatic infection.
Cell Microbiol, 19 (2).
ISSN 1462-5822
https://doi.org/10.1111/cmi.12646
SGUL Authors: Staines, Henry Michael
|
PDF
Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (1MB) | Preview |
|
|
PDF
Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (1MB) | Preview |
Abstract
Intracellular pathogens have evolved mechanisms to ensure their survival and development inside their host cells. Here, we show that glucose is a pivotal modulator of hepatic infection by the rodent malaria parasite Plasmodium berghei and that glucose uptake via the GLUT1 transporter is specifically enhanced in P. berghei-infected cells. We further show that ATP levels of cells containing developing parasites are decreased, which is known to enhance membrane GLUT1 activity. In addition, GLUT1 molecules are translocated to the membrane of the hepatic cell, increasing glucose uptake at later stages of infection. Chemical inhibition of GLUT1 activity leads to a decrease in glucose uptake and the consequent impairment of hepatic infection, both in vitro and in vivo. Our results reveal that changes in GLUT1 conformation and cellular localization seem to be part of an adaptive host response to maintain adequate cellular nutrition and energy levels, ensuring host cell survival and supporting P. berghei hepatic development.
Item Type: | Article | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Additional Information: | © 2016 The Authors Cellular Microbiology Published by John Wiley & Sons Ltd This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | ||||||||||||
Keywords: | Microbiology, 0605 Microbiology, 1108 Medical Microbiology | ||||||||||||
SGUL Research Institute / Research Centre: | Academic Structure > Infection and Immunity Research Institute (INII) | ||||||||||||
Journal or Publication Title: | Cell Microbiol | ||||||||||||
Article Number: | e12646 | ||||||||||||
ISSN: | 1462-5822 | ||||||||||||
Language: | eng | ||||||||||||
Publisher License: | Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0 | ||||||||||||
Projects: |
|
||||||||||||
PubMed ID: | 27404888 | ||||||||||||
Go to PubMed abstract | |||||||||||||
URI: | https://openaccess.sgul.ac.uk/id/eprint/108080 | ||||||||||||
Publisher's version: | https://doi.org/10.1111/cmi.12646 |
Statistics
Actions (login required)
![]() |
Edit Item |