Advancing, promoting and sharing knowledge of health through excellence in teaching, clinical practice and research into the prevention and treatment of illness

Pooling serum samples may lead to loss of potential biomarkers in SELDI-ToF MS proteomic profiling.

Sadiq, ST; Agranoff, D (2008) Pooling serum samples may lead to loss of potential biomarkers in SELDI-ToF MS proteomic profiling. PROTEOME SCIENCE, 6 (16). ISSN 1477-5956
SGUL Authors: Sadiq, Syed Tariq

["document_typename_application/pdf; charset=binary" not defined] Published Version
Download (285kB) | Preview


BACKGROUND: High throughput proteomic technology offers promise for the detection of disease biomarkers and proteomic signature patterns but biomarker discovery studies can be limited by cost factors when large sample size numbers are required. Pooling sera or plasma samples from disease cases potentially offers a solution to cost implications by reducing the standard errors of mass to charge values. Surface enhanced laser desorption/ionization time of flight (SELDI-ToF) mass spectra obtained from individual and pooled sera from invasive aspergillosis cases and controls were compared. RESULTS: Pooling resulted in 50% loss of peak clusters detected in individual samples. Overall, loss was greatest for low intensity clusters. Peak intensities and case:control intensity ratios, among clusters not lost, demonstrated good reproducibility. CONCLUSION: Pooling sera results in significant potential biomarker loss when using SELDI-ToF MS.

Item Type: Article
Additional Information: PubMed ID: 18513446 © 2008 Sadiq and Agranoff; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Biochemistry & Molecular Biology, PROSTATE-CANCER
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: PROTEOME SCIENCE
ISSN: 1477-5956
Related URLs:
1 June 2008Published
Web of Science ID: WOS:000256480400001
Download EPMC Full text (PDF)
Download EPMC Full text (HTML)
Publisher's version:

Actions (login required)

Edit Item Edit Item