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Genetic variation in Staphylococcus aureus surface and immune evasion genes is lineage associated: implications for vaccine design and host-pathogen interactions.

McCarthy, AJ; Lindsay, JA (2010) Genetic variation in Staphylococcus aureus surface and immune evasion genes is lineage associated: implications for vaccine design and host-pathogen interactions. BMC MICROBIOLOGY, 10 (173). ISSN 1471-2180
SGUL Authors: Lindsay, Jodi Anne

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BACKGROUND: S. aureus is a coloniser and pathogen of humans and mammals. Whole genome sequences of 58 strains of S. aureus in the public domain and data from multi-strain microarrays were compared to assess variation in the sequence of proteins known or putatively interacting with host. RESULTS: These included 24 surface proteins implicated in adhesion (ClfA, ClfB, Cna, Eap, Ebh, EbpS, FnBPA, FnBPB, IsaB, IsdA, IsdB, IsdH, SasB, SasC, SasD, SasF, SasG, SasH, SasK, SdrC, SdrD, SdrE, Spa and SraP) and 13 secreted proteins implicated in immune response evasion (Coa, Ecb, Efb, Emp, EsaC, EsxA, EssC, FLIPr, FLIPr like, Sbi, SCIN-B, SCIN-C, VWbp) located on the stable core genome. Many surface protein genes were missing or truncated, unlike immune evasion genes, and several distinct variants were identified. Domain variants were lineage specific. Unrelated lineages often possess the same sequence variant domains proving that horizontal transfer and recombination has contributed to their evolution. Surprisingly, sequenced strains from four animal S. aureus strains had surface and immune evasion proteins remarkably similar to those found in human strains, yet putative targets of these proteins vary substantially between different hosts. This suggests these proteins are not essential for virulence. However, the most variant protein domains were the putative functional regions and there is biological evidence that variants can be functional, arguing they do play a role. CONCLUSION: Surface and immune evasion genes are candidates for S. aureus vaccines, and their distribution and functionality is key. Vaccines should contain cocktails of antigens representing all variants or they will not protect against naturally occurring S. aureus populations.

Item Type: Article
Additional Information: PubMed ID: 20550675 © 2010 McCarthy and Lindsay; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Antigens, Bacterial, Bacterial Proteins, Gene Expression Regulation, Bacterial, Genetic Variation, Genome, Bacterial, Host-Pathogen Interactions, Protein Array Analysis, Staphylococcal Vaccines, Staphylococcus aureus, Science & Technology, Life Sciences & Biomedicine, Microbiology, FIBRONECTIN-BINDING PROTEIN, COMMUNITY-ACQUIRED MRSA, WALL-ASSOCIATED PROTEIN, FACTOR-B CLFB, METHICILLIN-RESISTANT, CLUMPING FACTOR, CELL-SURFACE, GENOME SEQUENCE, COMPLEMENT EVASION, NASAL CARRIAGE
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: BMC MICROBIOLOGY
ISSN: 1471-2180
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15 June 2010Published
Web of Science ID: WOS:000280802300001
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