SORA

Advancing, promoting and sharing knowledge of health through excellence in teaching, clinical practice and research into the prevention and treatment of illness

KV7 Channel Expression and Function Within Rat Mesenteric Endothelial Cells.

Baldwin, SN; Sandow, SL; Mondéjar-Parreño, G; Stott, JB; Greenwood, IA (2020) KV7 Channel Expression and Function Within Rat Mesenteric Endothelial Cells. Front Physiol, 11. p. 598779. ISSN 1664-042X https://doi.org/10.3389/fphys.2020.598779
SGUL Authors: Greenwood, Iain Andrew

[img]
Preview
PDF Published Version
Available under License Creative Commons Attribution.

Download (3MB) | Preview

Abstract

Background and Purpose: Arterial diameter is dictated by the contractile state of the vascular smooth muscle cells (VSMCs), which is modulated by direct and indirect inputs from endothelial cells (ECs). Modulators of KCNQ-encoded kV7 channels have considerable impact on arterial diameter and these channels are known to be expressed in VSMCs but not yet defined in ECs. However, expression of kV7 channels in ECs would add an extra level of vascular control. This study aims to characterize the expression and function of KV7 channels within rat mesenteric artery ECs. Experimental Approach: In rat mesenteric artery, KCNQ transcript and KV7 channel protein expression were determined via RT-qPCR, immunocytochemistry, immunohistochemistry and immunoelectron microscopy. Wire myography was used to determine vascular reactivity. Key Results: KCNQ transcript was identified in isolated ECs and VSMCs. KV7.1, KV7.4 and KV7.5 protein expression was determined in both isolated EC and VSMC and in whole vessels. Removal of ECs attenuated vasorelaxation to two structurally different KV7.2-5 activators S-1 and ML213. KIR2 blockers ML133, and BaCl2 also attenuated S-1 or ML213-mediated vasorelaxation in an endothelium-dependent process. KV7 inhibition attenuated receptor-dependent nitric oxide (NO)-mediated vasorelaxation to carbachol, but had no impact on relaxation to the NO donor, SNP. Conclusion and Implications: In rat mesenteric artery ECs, KV7.4 and KV7.5 channels are expressed, functionally interact with endothelial KIR2.x channels and contribute to endogenous eNOS-mediated relaxation. This study identifies KV7 channels as novel functional channels within rat mesenteric ECs and suggests that these channels are involved in NO release from the endothelium of these vessels.

Item Type: Article
Additional Information: Copyright © 2020 Baldwin, Sandow, Mondéjar-Parreño, Stott and Greenwood. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Keywords: KIR channel, KV7 channel, carbachol, endothelial cell, pharmacology, vascular biology
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Journal or Publication Title: Front Physiol
ISSN: 1664-042X
Language: eng
Dates:
DateEvent
7 December 2020Published
13 November 2020Accepted
Publisher License: Creative Commons: Attribution 4.0
Projects:
Project IDFunderFunder ID
FS/18/41/33762British Heart Foundationhttp://dx.doi.org/10.13039/501100000274
PubMed ID: 33364977
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/112765
Publisher's version: https://doi.org/10.3389/fphys.2020.598779

Actions (login required)

Edit Item Edit Item