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Whipworm genome and dual-species transcriptome analyses provide molecular insights into an intimate host-parasite interaction.

Foth, BJ; Tsai, IJ; Reid, AJ; Bancroft, AJ; Nichol, S; Tracey, A; Holroyd, N; Cotton, JA; Stanley, EJ; Zarowiecki, M; et al. Foth, BJ; Tsai, IJ; Reid, AJ; Bancroft, AJ; Nichol, S; Tracey, A; Holroyd, N; Cotton, JA; Stanley, EJ; Zarowiecki, M; Liu, JZ; Huckvale, T; Cooper, PJ; Grencis, RK; Berriman, M (2014) Whipworm genome and dual-species transcriptome analyses provide molecular insights into an intimate host-parasite interaction. Nat Genet, 46 (7). pp. 693-700. ISSN 1546-1718 https://doi.org/10.1038/ng.3010
SGUL Authors: Cooper, Philip John

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Abstract

Whipworms are common soil-transmitted helminths that cause debilitating chronic infections in man. These nematodes are only distantly related to Caenorhabditis elegans and have evolved to occupy an unusual niche, tunneling through epithelial cells of the large intestine. We report here the whole-genome sequences of the human-infective Trichuris trichiura and the mouse laboratory model Trichuris muris. On the basis of whole-transcriptome analyses, we identify many genes that are expressed in a sex- or life stage-specific manner and characterize the transcriptional landscape of a morphological region with unique biological adaptations, namely, bacillary band and stichosome, found only in whipworms and related parasites. Using RNA sequencing data from whipworm-infected mice, we describe the regulated T helper 1 (TH1)-like immune response of the chronically infected cecum in unprecedented detail. In silico screening identified numerous new potential drug targets against trichuriasis. Together, these genomes and associated functional data elucidate key aspects of the molecular host-parasite interactions that define chronic whipworm infection.

Item Type: Article
Additional Information: This is a post-peer-review, pre-copyedit version of an article published in Nature Genetics. The final authenticated version is available online at: http://dx.doi.org/10.1038/ng.3010
Keywords: Animals, Gene Expression Profiling, Genome, Helminth, Host-Parasite Interactions, Humans, Intestines, Male, Mice, Mice, Inbred C57BL, Phylogeny, Species Specificity, Th1 Cells, Trichuriasis, Trichuris, Intestines, Th1 Cells, Animals, Mice, Inbred C57BL, Humans, Mice, Trichuris, Trichuriasis, Gene Expression Profiling, Phylogeny, Species Specificity, Male, Genome, Helminth, Host-Parasite Interactions, Science & Technology, Life Sciences & Biomedicine, Genetics & Heredity, GENETICS & HEREDITY, GASTROINTESTINAL HELMINTH INFECTION, NEMATODE TRICHINELLA-SPIRALIS, TRICHURIS-MURIS, DOMAIN PROTEINS, DRAFT GENOME, DNASE II, RESISTANCE, GENES, METAANALYSIS, KARYOTYPE, 11 Medical And Health Sciences, 06 Biological Sciences, Developmental Biology
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: Nat Genet
ISSN: 1546-1718
Language: eng
Dates:
DateEvent
July 2014Published
15 June 2014Published Online
22 May 2014Accepted
Publisher License: Publisher's own licence
Projects:
Project IDFunderFunder ID
088862/Z/09/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
100290Wellcome Trusthttp://dx.doi.org/10.13039/100004440
WT100290MAWellcome Trusthttp://dx.doi.org/10.13039/100004440
100714Wellcome Trusthttp://dx.doi.org/10.13039/100004440
088862Wellcome Trusthttp://dx.doi.org/10.13039/100004440
098051Wellcome Trusthttp://dx.doi.org/10.13039/100004440
WT083620MAWellcome Trusthttp://dx.doi.org/10.13039/100004440
PubMed ID: 24929830
Web of Science ID: WOS:000338093800009
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/110549
Publisher's version: https://doi.org/10.1038/ng.3010

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