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Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation.

Vo, N; Anh Suong, DN; Yoshino, N; Yoshida, H; Cotterill, S; Yamaguchi, M (2017) Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation. Nucleic Acids Res, 45 (3). pp. 1233-1254. ISSN 1362-4962 https://doi.org/10.1093/nar/gkw1174
SGUL Authors: Cotterill, Susan Margaret

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Abstract

Both Mcm10 and HP1a are known to be required for DNA replication. However, underlying mechanism is not clarified yet especially for HP1. Knockdown of both HP1a and Mcm10 genes inhibited the progression of S phase in Drosophila eye imaginal discs. Proximity Ligation Assay (PLA) demonstrated that HP1a is in close proximity to DNA replication proteins including Mcm10, RFC140 and DNA polymerase ε 255 kDa subunit in S-phase. This was further confirmed by co-immunoprecipitation assay. The PLA signals between Mcm10 and HP1a are specifically observed in the mitotic cycling cells, but not in the endocycling cells. Interestingly, many cells in the posterior regions of eye imaginal discs carrying a double knockdown of Mcm10 and HP1a induced ectopic DNA synthesis and DNA damage without much of ectopic apoptosis. Therefore, the G1-S checkpoint may be affected by knockdown of both proteins. This event was also the case with other HP family proteins such as HP4 and HP6. In addition, both Mcm10 and HP1a are required for differentiation of photoreceptor cells R1, R6 and R7. Further analyses on several developmental genes involved in the photoreceptor cell differentiation suggest that a role of both proteins is mediated by regulation of the lozenge gene.

Item Type: Article
Additional Information: © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
Keywords: Animals, Animals, Genetically Modified, Cell Differentiation, Chromosomal Proteins, Non-Histone, DNA Polymerase II, DNA Replication, DNA-Binding Proteins, Drosophila Proteins, Drosophila melanogaster, Eye, Female, G1 Phase Cell Cycle Checkpoints, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, Genome, Insect, Male, Microscopy, Electron, Scanning, Minichromosome Maintenance Proteins, Nerve Tissue Proteins, Nuclear Proteins, Photoreceptor Cells, Invertebrate, Recombinant Proteins, Replication Protein C, Transcription Factors, Developmental Biology, 05 Environmental Sciences, 06 Biological Sciences, 08 Information And Computing Sciences
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Cell Sciences (INCCCS)
Journal or Publication Title: Nucleic Acids Res
ISSN: 1362-4962
Language: eng
Dates:
DateEvent
February 2017Published
28 November 2016Published Online
13 November 2016Accepted
Publisher License: Creative Commons: Attribution-Noncommercial 4.0
PubMed ID: 28180289
Web of Science ID: WOS:000397008000022
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/109680
Publisher's version: https://doi.org/10.1093/nar/gkw1174

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