Rattanapisit, K; Abdulheem, S; Chaikeawkaew, D; Kubera, A; Mason, HS; Ma, JK-C; Pavasant, P; Phoolcharoen, W
(2017)
Recombinant human osteopontin expressed in Nicotiana benthamiana stimulates osteogenesis related genes in human periodontal ligament cells.
Sci Rep, 7 (1).
p. 17358.
ISSN 2045-2322
https://doi.org/10.1038/s41598-017-17666-7
SGUL Authors: Ma, Julian
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Abstract
Tissue engineering aims to utilise biologic mediators to facilitate tissue regeneration. Several recombinant proteins have potential to mediate induction of bone production, however, the high production cost of mammalian cell expression impedes patient access to such treatments. The aim of this study is to produce recombinant human osteopontin (hOPN) in plants for inducing dental bone regeneration. The expression host was Nicotiana benthamiana using a geminiviral vector for transient expression. OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni affinity chromatography. Structural analysis indicated that plant-produced hOPN had a structure similar to commercial HEK cell-produced hOPN. Biological function of the plant-produced hOPN was also examined. Human periodontal ligament stem cells were seeded on an OPN-coated surface. The results indicated that cells could grow normally on plant-produced hOPN as compared to commercial HEK cell-produced hOPN determined by MTT assay. Interestingly, increased expression of osteogenic differentiation-related genes, including OSX, DMP1, and Wnt3a, was observed by realtime PCR. These results show the potential of plant-produced OPN to induce osteogenic differentiation of stem cells from periodontal ligament in vitro, and suggest a therapeutic strategy for bone regeneration in the future.
| Item Type: | Article | |||||||||
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| Additional Information: | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2017 | |||||||||
| SGUL Research Institute / Research Centre: | Academic Structure > Infection and Immunity Research Institute (INII) | |||||||||
| Journal or Publication Title: | Sci Rep | |||||||||
| ISSN: | 2045-2322 | |||||||||
| Language: | eng | |||||||||
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| Publisher License: | Creative Commons: Attribution 4.0 | |||||||||
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| PubMed ID: | 29229947 | |||||||||
| Web of Science ID: | WOS:000417570500077 | |||||||||
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| URI: | https://openaccess.sgul.ac.uk/id/eprint/109646 | |||||||||
| Publisher's version: | https://doi.org/10.1038/s41598-017-17666-7 |
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