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Whole-genome sequencing illuminates the evolution and spread of multidrug-resistant tuberculosis in Southwest Nigeria.

Senghore, M; Otu, J; Witney, A; Gehre, F; Doughty, EL; Kay, GL; Butcher, P; Salako, K; Kehinde, A; Onyejepu, N; et al. Senghore, M; Otu, J; Witney, A; Gehre, F; Doughty, EL; Kay, GL; Butcher, P; Salako, K; Kehinde, A; Onyejepu, N; Idigbe, E; Corrah, T; de Jong, B; Pallen, MJ; Antonio, M (2017) Whole-genome sequencing illuminates the evolution and spread of multidrug-resistant tuberculosis in Southwest Nigeria. PLoS One, 12 (9). e0184510. ISSN 1932-6203 https://doi.org/10.1371/journal.pone.0184510
SGUL Authors: Witney, Adam Austin

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Abstract

Nigeria has an emerging problem with multidrug-resistant tuberculosis (MDR-TB). Whole-genome sequencing was used to understand the epidemiology of tuberculosis and genetics of multi-drug resistance among patients from two tertiary referral centers in Southwest Nigeria. In line with previous molecular epidemiology studies, most isolates of Mycobacterium tuberculosis from this dataset belonged to the Cameroon clade within the Euro-American lineage. Phylogenetic analysis showed this clade was undergoing clonal expansion in this region, and suggests that it was involved in community transmission of sensitive and multidrug-resistant tuberculosis. Five patients enrolled for retreatment were infected with pre-extensively drug resistant (pre-XDR) due to fluoroquinolone resistance in isolates from the Cameroon clade. In all five cases resistance was conferred through a mutation in the gyrA gene. In some patients, genomic changes occurred in bacterial isolates during the course of treatment that potentially led to decreased drug susceptibility. We conclude that inter-patient transmission of resistant isolates, principally from the Cameroon clade, contributes to the spread of MDR-TB in this setting, underscoring the urgent need to curb the spread of multi-drug resistance in this region.

Item Type: Article
Additional Information: © 2017 Senghore et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: General Science & Technology, MD Multidisciplinary
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: PLoS One
ISSN: 1932-6203
Language: eng
Dates:
DateEvent
19 September 2017Published
27 August 2017Accepted
Publisher License: Creative Commons: Attribution 4.0
Projects:
Project IDFunderFunder ID
2007.41700.007European and Developing Countries Clinical Trials Partnershiphttp://dx.doi.org/10.13039/501100001713
PubMed ID: 28926571
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/109154
Publisher's version: https://doi.org/10.1371/journal.pone.0184510

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