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DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus.

Cooper, LP; Roberts, GA; White, JH; Luyten, YA; Bower, EKM; Morgan, RD; Roberts, RJ; Lindsay, JA; Dryden, DTF (2017) DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus. Nucleic Acids Res, 45 (6). pp. 3395-3406. ISSN 1362-4962 https://doi.org/10.1093/nar/gkx067
SGUL Authors: Lindsay, Jodi Anne

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Abstract

Staphylococcus aureus displays a clonal population structure in which horizontal gene transfer between different lineages is extremely rare. This is due, in part, to the presence of a Type I DNA restriction–modification (RM) system given the generic name of Sau1, which maintains different patterns of methylation on specific target sequences on the genomes of different lineages. We have determined the target sequences recognized by the Sau1 Type I RM systems present in a wide range of the most prevalent S. aureus lineages and assigned the sequences recognized to particular target recognition domains within the RM enzymes. We used a range of biochemical assays on purified enzymes and single molecule real-time sequencing on genomic DNA to determine these target sequences and their patterns of methylation. Knowledge of the main target sequences for Sau1 will facilitate the synthesis of new vectors for transformation of the most prevalent lineages of this ‘untransformable’ bacterium.

Item Type: Article
Additional Information: © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Developmental Biology, 05 Environmental Sciences, 06 Biological Sciences, 08 Information And Computing Sciences
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: Nucleic Acids Res
ISSN: 1362-4962
Language: eng
Dates:
DateEvent
7 April 2017Published
9 February 2017Published Online
3 February 2017Accepted
Publisher License: Creative Commons: Attribution 4.0
Projects:
Project IDFunderFunder ID
GR080463MAWellcome Trusthttp://dx.doi.org/10.13039/100004440
090288/Z/09/ZAWellcome Trusthttp://dx.doi.org/10.13039/100004440
PubMed ID: 28180279
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/108590
Publisher's version: https://doi.org/10.1093/nar/gkx067

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