Advancing, promoting and sharing knowledge of health through excellence in teaching, clinical practice and research into the prevention and treatment of illness

A genetic analysis of Trichuris trichiura and Trichuris suis from Ecuador.

Meekums, H; Hawash, MB; Sparks, AM; Oviedo, Y; Sandoval, C; Chico, ME; Stothard, JR; Cooper, PJ; Nejsum, P; Betson, M (2015) A genetic analysis of Trichuris trichiura and Trichuris suis from Ecuador. Parasit Vectors, 8 (168). ISSN 1756-3305
SGUL Authors: Cooper, Philip John

["document_typename_cannot open `/data/SGUL/sgul/eprints3/archives/sgul/documents/disk0/00/10/74/46/01/A' (No such file or directory) cannot open `genetic' (No such file or directory) cannot open `analysis' (No such file or directory) cannot open `of' (No such file or direct" not defined] Published Version
Available under License Creative Commons Attribution.

Download (504kB) | Preview


BACKGROUND: Since the nematodes Trichuris trichiura and T. suis are morphologically indistinguishable, genetic analysis is required to assess epidemiological cross-over between people and pigs. This study aimed to clarify the transmission biology of trichuriasis in Ecuador. FINDINGS: Adult Trichuris worms were collected during a parasitological survey of 132 people and 46 pigs in Esmeraldas Province, Ecuador. Morphometric analysis of 49 pig worms and 64 human worms revealed significant variation. In discriminant analysis morphometric characteristics correctly classified male worms according to host species. In PCR-RFLP analysis of the ribosomal Internal Transcribed Spacer (ITS-2) and 18S DNA (59 pig worms and 82 human worms), nearly all Trichuris exhibited expected restriction patterns. However, two pig-derived worms showed a "heterozygous-type" ITS-2 pattern, with one also having a "heterozygous-type" 18S pattern. Phylogenetic analysis of the mitochondrial large ribosomal subunit partitioned worms by host species. Notably, some Ecuadorian T. suis clustered with porcine Trichuris from USA and Denmark and some with Chinese T. suis. CONCLUSION: This is the first study in Latin America to genetically analyse Trichuris parasites. Although T. trichiura does not appear to be zoonotic in Ecuador, there is evidence of genetic exchange between T. trichiura and T. suis warranting more detailed genetic sampling.

Item Type: Article
Additional Information: © 2015 The Authors. Made available under the terms of the Creative Commons Attribution 4.0 Unported licence (CC BY 4.0)
Keywords: Trichuris, Whipworm, Human, Pig, Ecuador, Zoonosis, Phylogenetics, Mycology & Parasitology, 1108 Medical Microbiology, 1117 Public Health And Health Services
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: Parasit Vectors
ISSN: 1756-3305
Language: eng
19 March 2015Published
Publisher License: Creative Commons: Attribution 4.0
Project IDFunderFunder ID
088862/Z/09/ZWellcome TrustUNSPECIFIED
PubMed ID: 25889461
Web of Science ID: WOS:000351902200001
Go to PubMed abstract
Publisher's version:

Actions (login required)

Edit Item Edit Item