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Resident phenotypically modulated vascular smooth muscle cells in healthy human arteries.

Harhun, MI; Huggins, CL; Ratnasingham, K; Raje, D; Moss, RF; Szewczyk, K; Vasilikostas, G; Greenwood, IA; Khong, TK; Wan, A; et al. Harhun, MI; Huggins, CL; Ratnasingham, K; Raje, D; Moss, RF; Szewczyk, K; Vasilikostas, G; Greenwood, IA; Khong, TK; Wan, A; Reddy, M (2012) Resident phenotypically modulated vascular smooth muscle cells in healthy human arteries. Journal of Cellular and Molecular Medicine, 16 (11). pp. 2802-2812. ISSN 1582-4934 https://doi.org/10.1111/j.1582-4934.2012.01609.x
SGUL Authors: Greenwood, Iain Andrew Khong, Teck Kean Harhun, Maksym

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Abstract

Vascular interstitial cells (VICs) are non-contractile cells with filopodia previously described in healthy blood vessels of rodents and their function remains unknown. The objective of this study was to identify VICs in human arteries and to ascertain their role. VICs were identified in the wall of human gastro-omental arteries using transmission electron microscopy. Isolated VICs showed ability to form new and elongate existing filopodia and actively change body shape. Most importantly sprouting VICs were also observed in cell dispersal. RT-PCR performed on separately collected contractile vascular smooth muscle cells (VSMCs) and VICs showed that both cell types expressed the gene for smooth muscle myosin heavy chain (SM-MHC). Immunofluorescent labelling showed that both VSMCs and VICs had similar fluorescence for SM-MHC and αSM-actin, VICs, however, had significantly lower fluorescence for smoothelin, myosin light chain kinase, h-calponin and SM22α. It was also found that VICs do not have cytoskeleton as rigid as in contractile VSMCs. VICs express number of VSMC-specific proteins and display features of phenotypically modulated VSMCs with increased migratory abilities. VICs, therefore represent resident phenotypically modulated VSMCs that are present in human arteries under normal physiological conditions.

Item Type: Article
Additional Information: © 2012 The Authors Journal of Cellular and Molecular Medicine. © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd. Open Access. The published version made available here with permission from the publisher.
Keywords: Actins, Arteries, Biological Markers, Calcium-Binding Proteins, Cytoskeleton, Female, Humans, Male, Microfilament Proteins, Microscopy, Electron, Transmission, Middle Aged, Muscle Proteins, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, Myosin-Light-Chain Kinase, Phenotype, vascular interstitial cell, vascular smooth muscle cell, human, phenotypically modulated vascular smooth muscle cells, gastro-omental arteries, filopodia, budding, Science & Technology, Life Sciences & Biomedicine, Cell Biology, Medicine, Research & Experimental, Research & Experimental Medicine, CELL BIOLOGY, MEDICINE, RESEARCH & EXPERIMENTAL, RABBIT PORTAL-VEIN, CAJAL-LIKE CELLS, INTERSTITIAL-CELLS, PROGENITOR CELLS, GASTROINTESTINAL-TRACT, CONTRACTILE PHENOTYPE, IN-VIVO, BLOOD-VESSELS, C-KIT, TELOCYTES, Biochemistry & Molecular Biology, 0304 Medicinal And Biomolecular Chemistry, 0601 Biochemistry And Cell Biology, 1103 Clinical Sciences
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Vascular (INCCVA)
Journal or Publication Title: Journal of Cellular and Molecular Medicine
ISSN: 1582-4934
Language: eng
Dates:
DateEvent
November 2012Published
Projects:
Project IDFunderFunder ID
FS/06/077British Heart FoundationUNSPECIFIED
FS/06/077/22004British Heart FoundationUNSPECIFIED
PubMed ID: 22862785
Web of Science ID: WOS:000310555200024
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/107386
Publisher's version: https://doi.org/10.1111/j.1582-4934.2012.01609.x

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