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Human CD4 memory T cells can become CD4+IL-9+ T cells.

Putheti, P; Awasthi, A; Popoola, J; Gao, W; Strom, TB (2010) Human CD4 memory T cells can become CD4+IL-9+ T cells. PLoS One, 5 (1). ISSN 1932-6203
SGUL Authors: Popoola, Joyce

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BACKGROUND: IL-9 is a growth factor for T- and mast-cells that is secreted by human Th2 cells. We recently reported that IL-4+TGF-beta directs mouse CD4(+)CD25(-)CD62L(+) T cells to commit to inflammatory IL-9 producing CD4(+) T cells. METHODOLOGY/PRINCIPAL FINDINGS: Here we show that human inducible regulatory T cells (iTregs) also express IL-9. IL-4+TGF-beta induced higher levels of IL-9 expression in plate bound-anti-CD3 mAb (pbCD3)/soluble-anti-CD28 mAb (sCD28) activated human resting memory CD4(+)CD25(-)CD45RO(+) T cells as compared to naïve CD4(+)CD25(-)CD45RA(+) T cells. In addition, as compared to pbCD3/sCD28 plus TGF-beta stimulation, IL-4+TGF-beta stimulated memory CD4(+)CD25(-)CD45RO(+) T cells expressed reduced FOXP3 protein. As analyzed by pre-amplification boosted single-cell real-time PCR, human CD4(+)IL-9(+) T cells expressed GATA3 and RORC, but not IL-10, IL-13, IFNgamma or IL-17A/F. Attempts to optimize IL-9 production by pbCD3/sCD28 and IL-4+TGF-beta stimulated resting memory CD4(+) T cells demonstrated that the addition of IL-1beta, IL-12, and IL-21 further enhance IL-9 production. CONCLUSIONS/SIGNIFICANCE: Taken together these data show both the differences and similarities between mouse and human CD4(+)IL9(+) T cells and reaffirm the powerful influence of inflammatory cytokines to shape the response of activated CD4(+) T cells to antigen.

Item Type: Article
Additional Information: © 2010 Putheti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Antigens, CD4, CD4-Positive T-Lymphocytes, Cytokines, Enzyme-Linked Immunosorbent Assay, Humans, Immunologic Memory, Interleukin-9, Polymerase Chain Reaction, General Science & Technology, MD Multidisciplinary
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Clinical Education (INMECE )
Journal or Publication Title: PLoS One
Article Number: e8706
ISSN: 1932-6203
Language: eng
14 January 2010Published
Project IDFunderFunder ID
PubMed ID: 20090929
Web of Science ID: WOS:000273714600009
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