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A novel liver specific isoform of the rat LAR transcript is expressed as a truncated isoform encoded from a 5 ' UTR located within intron 11

Welham, SJ; Clark, AJ; Salter, AM (2009) A novel liver specific isoform of the rat LAR transcript is expressed as a truncated isoform encoded from a 5 ' UTR located within intron 11. BMC MOLECULAR BIOLOGY, 10 (30). ISSN 1471-2199
SGUL Authors: Clark, Adrian John L

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Background: The leukocyte common antigen related receptor (LAR) protein has been shown to modulate the signal transduction of a number of different growth factors, including insulin and insulin-like growth factor 1. Splice variants exhibit differing roles and are expressed according to tissue type and developmental stage. Results: Using 5'RACE, we identified a 5'UTR within intron 11 of the rat LAR gene. We demonstrated that this gives rise to a novel isoform of the LAR transcript encoded from the identified region within intron 11. By priming across the site from exon 11 to exon 15 we show that the novel 5'UTR is not represented in the full-length transcript and thus, it produces a truncated form of the LAR mRNA. We examined the tissue distribution of this novel isoform and found it to be exclusively expressed in liver. We additionally identified a liver specific 150 kDa band with western blotting which we propose may represent the protein product of the novel transcript. Luciferase assays showed the region immediately upstream of the 5'UTR to possesses considerable promoter activity and that this may be conferred by the presence of a number of putative binding sites for liver enriched transcription factors. Conclusion: In summary, we describe a novel, liver specific, truncated isoform of the LAR transcript transcribed under the control of an intronic promoter, potentially representing a previously unidentified modulator of hepatic insulin signalling.

Item Type: Article
Additional Information: Copyright: 2009 Welham et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Keywords: 5' Untranslated Regions, Animals, Base Sequence, Gene Expression Regulation, Enzymologic, Introns, Liver, Promoter Regions, Genetic, Protein Isoforms, Rats, Receptor-Like Protein Tyrosine Phosphatases, Class 2, Sequence Alignment, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, BIOCHEMISTRY & MOLECULAR BIOLOGY, PROTEIN-TYROSINE-PHOSPHATASE, INSULIN-RECEPTOR, SIGNALING PATHWAYS, NEURITE OUTGROWTH, DEPHOSPHORYLATION, PEPTIDES, CLEAVAGE, GROWTH, OVEREXPRESSION, IDENTIFICATION, Developmental Biology, 0601 Biochemistry And Cell Biology, 1101 Medical Biochemistry And Metabolomics, 1113 Ophthalmology And Optometry
Journal or Publication Title: BMC MOLECULAR BIOLOGY
ISSN: 1471-2199
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8 April 2009Published
Web of Science ID: WOS:000265849700002
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