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Multiple Colonization with S. pneumoniae before and after Introduction of the Seven-Valent Conjugated Pneumococcal Polysaccharide Vaccine

Brugger, SD; Frey, P; Aebi, S; Hinds, J; Mühlemann, K (2010) Multiple Colonization with S. pneumoniae before and after Introduction of the Seven-Valent Conjugated Pneumococcal Polysaccharide Vaccine. PLOS ONE, 5 (7). e11638 (1) - e11638 (8). ISSN 1932-6203 https://doi.org/10.1371/journal.pone.0011638
SGUL Authors: Hinds, Jason

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Abstract

Background: Simultaneous carriage of more than one strain of Streptococcus pneumoniae promotes horizontal gene transfer events and may lead to capsule switch and acquisition of antibiotic resistance. We studied the epidemiology of cocolonization with S. pneumoniae before and after introduction of the seven-valent conjugated pneumococcal vaccine(PCV7). Methodology: Nasopharyngeal swabs (n 1120) were collected from outpatients between 2004 and 2009 within an ongoing nationwide surveillance program. Cocolonization was detected directly from swabs by restriction fragment length polymorphism (RFLP) analysis. Serotypes were identified by agglutination, multiplex PCR and microarray. Principal Findings: Rate of multiple colonization remained stable up to three years after PCV7 introduction. Cocolonization was associated with serotypes of low carriage prevalence in the prevaccine era. Pneumococcal colonization density was higher in cocolonized samples and cocolonizing strains were present in a balanced ratio (median 1.38). Other characteristics of cocolonization were a higher frequency at young age, but no association with recurrent acute otitis media, recent antibiotic exposure, day care usage and PCV7 vaccination status. Conclusions: Pneumococcal cocolonization is dominated by serotypes of low carriage prevalence in the prevaccine era, which coexist in the nasopharynx. Emergence of such previously rare serotypes under vaccine selection pressure may promote cocolonization in the future.

Item Type: Article
Additional Information: Copyright: 2010 Brugger et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Adolescent, Adult, Child, Child, Preschool, Female, Humans, Male, Middle Aged, Nasopharynx, Pneumococcal Infections, Pneumococcal Vaccines, Serotyping, Streptococcus pneumoniae, Vaccines, Conjugate, Young Adult, Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, MULTIDISCIPLINARY SCIENCES, STREPTOCOCCUS-PNEUMONIAE, STAPHYLOCOCCUS-AUREUS, NASOPHARYNGEAL COLONIZATION, UNITED-STATES, SEROTYPE 19A, CHILDREN, DISEASE, CARRIAGE, PCR, POPULATION, General Science & Technology, MD Multidisciplinary
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: PLOS ONE
ISSN: 1932-6203
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Dates:
DateEvent
16 July 2010Published
Web of Science ID: WOS:000279980800022
URI: https://openaccess.sgul.ac.uk/id/eprint/107070
Publisher's version: https://doi.org/10.1371/journal.pone.0011638

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