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Characterization of VRC01, a potent and broadly neutralizing anti-HIV mAb, produced in transiently and stably transformed tobacco

Teh, AY; Maresch, D; Klein, K; Ma, JK (2014) Characterization of VRC01, a potent and broadly neutralizing anti-HIV mAb, produced in transiently and stably transformed tobacco. PLANT BIOTECHNOLOGY JOURNAL, 12 (3). 300 - 311. ISSN 1467-7644 https://doi.org/10.1111/pbi.12137
SGUL Authors: Teh, Yi Hui

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Abstract

The proposed clinical trial in Africa of VRC01, a potent broadly neutralizing antibody (bNAb) capable of neutralizing 91% of known HIV-1 isolates, raises concerns about testing a treatment which will be too expensive to be accessible by the most important target population, the poor in under-developed regions such as sub-Saharan Africa. Here, we report the expression of VRC01 in plants as an economic alternative to conventional mammalian-cell-based production platforms. The heavy and light chain genes of VRC01 were cloned onto a single vector, pTRAk.2, which was transformed into Nicotiana benthamiana or Nicotiana tabacum using transient and stable expression production systems respectively. VRC01 has been successfully expressed transiently in plants with expression level of approximately 80 mg antibody/kg; stable transgenic lines expressing up to 100 mg antibody/kg were also obtained. Plant-produced VRC01 from both systems showed a largely homogeneous N-glycosylation profile with a single dominant glycoform. The binding kinetics to gp120 IIIB (approximately 1 nM), neutralization of HIV-1 BaL or a panel of 10 VRC01-sensitive HIV-1 Env pseudoviruses of VRC01 produced in transient and stable plants were also consistent with VRC01 from HEK cells.

Item Type: Article
Additional Information: © 2013 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology, The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: HIV-1 broadly neutralizing antibody, VRC01, pTRAk.2, stable transgenics, transient expression, Science & Technology, Life Sciences & Biomedicine, Biotechnology & Applied Microbiology, Plant Sciences, BIOTECHNOLOGY & APPLIED MICROBIOLOGY, PLANT SCIENCES, VRC01, HIV-1 broadly neutralizing antibody, transient expression, stable transgenics, pTRAk, 2, ACTIVE ANTIRETROVIRAL THERAPY, TRANSGENIC PLANTS, NICOTIANA-BENTHAMIANA, HETEROLOGOUS PROTEINS, MONOCLONAL-ANTIBODIES, EXPRESSION, COMBINATION, AGROBACTERIUM, GENERATION, STRATEGIES
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: PLANT BIOTECHNOLOGY JOURNAL
ISSN: 1467-7644
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Dates:
DateEvent
1 April 2014Published
Web of Science ID: WOS:000333027500003
URI: https://openaccess.sgul.ac.uk/id/eprint/107033
Publisher's version: https://doi.org/10.1111/pbi.12137

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