Advancing, promoting and sharing knowledge of health through excellence in teaching, clinical practice and research into the prevention and treatment of illness

Basal body stability and ciliogenesis requires the conserved component Poc1

Pearson, CG; Osborn, DP; Giddings, TH; Beales, PL; Winey, M (2009) Basal body stability and ciliogenesis requires the conserved component Poc1. JOURNAL OF CELL BIOLOGY, 187 (6). 904 - 920 (16). ISSN 0021-9525
SGUL Authors: Osborn, Daniel Peter Sayer

["document_typename_application/pdf; charset=binary" not defined] Published Version
Download (6MB) | Preview


Centrioles are the foundation for centrosome and cilia formation. The biogenesis of centrioles is initiated by an assembly mechanism that first synthesizes the ninefold symmetrical cartwheel and subsequently leads to a stable cylindrical microtubule scaffold that is capable of withstanding microtubule-based forces generated by centrosomes and cilia. We report that the conserved WD40 repeat domain–containing cartwheel protein Poc1 is required for the structural maintenance of centrioles in Tetrahymena thermophila. Furthermore, human Poc1B is required for primary ciliogenesis, and in zebrafish, DrPoc1B knockdown causes ciliary defects and morphological phenotypes consistent with human ciliopathies. T. thermophila Poc1 exhibits a protein incorporation profile commonly associated with structural centriole components in which the majority of Poc1 is stably incorporated during new centriole assembly. A second dynamic population assembles throughout the cell cycle. Our experiments identify novel roles for Poc1 in centriole stability and ciliogenesis.

Item Type: Article
Additional Information: Copyright © 2009 Pearson et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at
Keywords: Amino Acid Motifs, Animals, Cell Line, Cell Movement, Centrioles, Cilia, Humans, Microfilament Proteins, Protein Structure, Tertiary, Protozoan Proteins, RNA Interference, Recombinant Fusion Proteins, Repetitive Sequences, Amino Acid, Temperature, Tetrahymena thermophila, Time Factors, Transfection, Zebrafish, Zebrafish Proteins, Science & Technology, Life Sciences & Biomedicine, Cell Biology, CELL BIOLOGY, TETRAHYMENA-THERMOPHILA, CENTROSOME DUPLICATION, ALPHA-TUBULIN, CENTRIOLE DUPLICATION, PRIMARY CILIUM, GAMMA-TUBULIN, C-ELEGANS, EPITHELIAL-CELLS, EPSILON-TUBULIN, 9-FOLD SYMMETRY
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Cell Sciences (INCCCS)
Journal or Publication Title: JOURNAL OF CELL BIOLOGY
ISSN: 0021-9525
Related URLs:
14 December 2009Published
Web of Science ID: WOS:000272702500015
Download EPMC Full text (PDF)
Download EPMC Full text (HTML)
Publisher's version:

Actions (login required)

Edit Item Edit Item