SORA

Advancing, promoting and sharing knowledge of health through excellence in teaching, clinical practice and research into the prevention and treatment of illness

Inhibition of pre-ischeamic conditioning in the mouse caudate brain slice by NMDA- or adenosine A(1) receptor antagonists

Chauhan, NK; Young, AM; Gibson, CL; Davidson, C (2013) Inhibition of pre-ischeamic conditioning in the mouse caudate brain slice by NMDA- or adenosine A(1) receptor antagonists. EUROPEAN JOURNAL OF PHARMACOLOGY, 698 (1-3). 322 - 329. ISSN 0014-2999 https://doi.org/10.1016/j.ejphar.2012.10.021
SGUL Authors: Davidson, Colin

[img]
Preview
["document_typename_application/pdf; charset=binary" not defined] Published Version
Available under License St George's repository terms & conditions.

Download (666kB) | Preview

Abstract

Evidence suggests that pre-ischeamic conditioning (PIC) offers protection against a subsequent ischeamic event. Although some brain areas such as the hippocampus have received much attention, the receptor mechanisms of PIC in other brain regions are unknown. We have previously shown that 10 min oxygen and glucose deprivation (OGD) evokes tolerance to a second OGD event in the caudate. Here we further examine the effect of length of conditioning event on the second OGD event. Caudate mouse brain slices were superfused with artificial cerebro-spinal fluid (aCSF) bubbled with 95%O2/5%CO2. OGD was achieved by reducing the aCSF glucose concentration and by bubbling with 95%N2/5%CO2. After approximately 5 min OGD a large dopamine efflux was observed, presumably caused by anoxic depolarisation. On applying a second OGD event, 60 min later, dopamine efflux was delayed and reduced. We first examined the effect of varying the length of the conditioning event from 5 to 40 min and found tolerance to PIC increased with increasing duration of conditioning. We then examined the receptor mechanism(s) underlying PIC. We found that pre-incubation with either MK-801 or 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) reduced tolerance to the second OGD event. These data suggest that either N-methyl-d-aspartate (NMDA) or adenosine A1 receptor activation evokes PIC in the mouse caudate.

Item Type: Article
Additional Information: © 2012 Elsevier B.V. Open access under CC BY license
Keywords: Adenosine A1 Receptor Antagonists, Animals, Brain Ischemia, Caudate Nucleus, Dizocilpine Maleate, Dopamine, Glucose, Heart Arrest, Ischemic Preconditioning, Male, Mice, Mice, Inbred C57BL, Oxygen, Receptor, Adenosine A1, Receptors, N-Methyl-D-Aspartate, Signal Transduction, Tetrazolium Salts, Xanthines, Science & Technology, Life Sciences & Biomedicine, Pharmacology & Pharmacy, Dopamine, Voltammetry, MK-801, DPCPX, TTC, HPLC, IN-VITRO MODEL, STRIATAL DOPAMINE RELEASE, ISCHEMIC TOLERANCE, CEREBRAL-ISCHEMIA, HIPPOCAMPAL SLICES, PRECONDITIONING NEUROPROTECTION, NEOSTRIATAL ISCHEMIA, GERBIL HIPPOCAMPUS, K-ATP(+) CHANNEL, REAL-TIME
SGUL Research Institute / Research Centre: Academic Structure > Institute of Medical & Biomedical Education (IMBE)
Academic Structure > Institute of Medical & Biomedical Education (IMBE) > Centre for Biomedical Education (INMEBE)
Journal or Publication Title: EUROPEAN JOURNAL OF PHARMACOLOGY
ISSN: 0014-2999
Related URLs:
Dates:
DateEvent
5 January 2013Published
Web of Science ID: WOS:000314616200041
Download EPMC Full text (HTML)
URI: https://openaccess.sgul.ac.uk/id/eprint/102144
Publisher's version: https://doi.org/10.1016/j.ejphar.2012.10.021

Actions (login required)

Edit Item Edit Item