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In vitro studies of amikacin-loaded human carrier erythrocytes

Gutiérrez Millán, C; Bax, BE; Castañeda, AZ; Marinero, ML; Lanao, JM (2008) In vitro studies of amikacin-loaded human carrier erythrocytes. TRANSLATIONAL RESEARCH, 152 (2). 59 - 66. ISSN 1931-5244
SGUL Authors: Bax, Bridget Elizabeth

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Erythrocyte-encapsulated antibiotics have the potential to provide an effective therapy against intracellular pathogens. The advantages over the administration of free antibiotics include a lower systemic dose, decreased toxicity, a sustained delivery of the antibiotic at higher concentrations to the intracellular site of pathogen replication, and increased efficacy. In this study, the encapsulation of amikacin by human carrier erythrocytes prepared using a hypo-osmotic dialysis was investigated. The effects of the initial amikacin dialysis concentration and hypo-osmotic dialysis time on the encapsulation efficiency of amikacin were determined, and the osmotic fragility and hematologic parameters of amikacin-loaded carrier erythrocytes were measured. The efficiency of amikacin entrapment by carrier erythrocytes was dependent on the initial dialysis concentration of the drug. Statistically significant differences in the osmotic fragility profiles between control and carrier erythrocytes were observed, which were dependent on the hypo-osmotic dialysis time and on the dialysis concentration of amikacin. Mean hematologic parameters were evaluated and compared with unloaded, native erythrocytes; the mean corpuscular volume (MCV) of amikacin-loaded carrier erythrocytes was statistically significant smaller. Amikacin demonstrated a sustained release from loaded erythrocytes over a 48-h period, which suggests a potential use of the erythrocyte as a slow systemic-release system for antibiotics.

Item Type: Article
Additional Information: NOTICE: this is the author’s version of a work that was accepted for publication in TRANSLATIONAL RESEARCH Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in TRANSLATIONAL RESEARCH [152, 2, (Aug 2008)]
Keywords: Amikacin, Dialysis, Drug Carriers, Erythrocytes, Hemoglobins, Hemolysis, Humans, Osmotic Fragility, Time Factors, Science & Technology, Life Sciences & Biomedicine, Medical Laboratory Technology, Medicine, General & Internal, Medicine, Research & Experimental, General & Internal Medicine, Research & Experimental Medicine, RED-BLOOD-CELLS, ADENOSINE-DEAMINASE, DIALYSIS, MACROPHAGES, ENTRAPMENT, DELIVERY, ENCAPSULATION, PROTEINS, SURVIVAL, THERAPY
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Cell Sciences (INCCCS)
Journal or Publication Title: TRANSLATIONAL RESEARCH
ISSN: 1931-5244
Related URLs:
1 August 2008Published
Web of Science ID: WOS:000259283700003
Publisher's version:

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