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Probing Host Pathogen Cross-Talk by Transcriptional Profiling of Both Mycobacterium tuberculosis and Infected Human Dendritic Cells and Macrophages.

Tailleux, L; Waddell, SJ; Pelizzola, M; Mortellaro, A; Withers, M; Tanne, A; Castagnoli, PR; Gicquel, B; Stoker, NG; Butcher, PD; et al. Tailleux, L; Waddell, SJ; Pelizzola, M; Mortellaro, A; Withers, M; Tanne, A; Castagnoli, PR; Gicquel, B; Stoker, NG; Butcher, PD; Foti, M; Neyrolles, O (2008) Probing Host Pathogen Cross-Talk by Transcriptional Profiling of Both Mycobacterium tuberculosis and Infected Human Dendritic Cells and Macrophages. PLOS ONE, 3 (1). e1403. ISSN 1932-6203 https://doi.org/10.1371/journal.pone.0001403
SGUL Authors: Butcher, Philip David

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Abstract

BACKGROUND: Transcriptional profiling using microarrays provides a unique opportunity to decipher host pathogen cross-talk on the global level. Here, for the first time, we have been able to investigate gene expression changes in both Mycobacterium tuberculosis, a major human pathogen, and its human host cells, macrophages and dendritic cells. METHODOLOGY/PRINCIPAL FINDINGS: In addition to common responses, we could identify eukaryotic and microbial transcriptional signatures that are specific to the cell type involved in the infection process. In particular M. tuberculosis shows a marked stress response when inside dendritic cells, which is in accordance with the low permissivity of these specialized phagocytes to the tubercle bacillus and to other pathogens. In contrast, the mycobacterial transcriptome inside macrophages reflects that of replicating bacteria. On the host cell side, differential responses to infection in macrophages and dendritic cells were identified in genes involved in oxidative stress, intracellular vesicle trafficking and phagosome acidification. CONCLUSIONS/SIGNIFICANCE: This study provides the proof of principle that probing the host and the microbe transcriptomes simultaneously is a valuable means to accessing unique information on host pathogen interactions. Our results also underline the extraordinary plasticity of host cell and pathogen responses to infection, and provide a solid framework to further understand the complex mechanisms involved in immunity to M. tuberculosis and in mycobacterial adaptation to different intracellular environments.

Item Type: Article
Additional Information: PubMed ID: 18167562 ©2008 Tailleux et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Dendritic Cells, Gene Expression Profiling, Humans, Macrophages, Mycobacterium tuberculosis, Transcription, Genetic, Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, INNATE IMMUNE-RESPONSES, GENE-EXPRESSION, NITRIC-OXIDE, LISTERIA-MONOCYTOGENES, REACTIVE OXYGEN, IN-VIVO, PULMONARY TUBERCULOSIS, ALVEOLAR MACROPHAGES, MICROARRAY ANALYSIS, HYPOXIC RESPONSE
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: PLOS ONE
ISSN: 1932-6203
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Dates:
DateEvent
2 January 2008Published
Web of Science ID: WOS:000260468900031
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URI: https://openaccess.sgul.ac.uk/id/eprint/82
Publisher's version: https://doi.org/10.1371/journal.pone.0001403

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