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A BLOC-1-AP-3 super-complex sorts a cis-SNARE complex into endosome-derived tubular transport carriers.

Bowman, SL; Le, L; Zhu, Y; Harper, DC; Sitaram, A; Theos, AC; Sviderskaya, EV; Bennett, DC; Raposo-Benedetti, G; Owen, DJ; et al. Bowman, SL; Le, L; Zhu, Y; Harper, DC; Sitaram, A; Theos, AC; Sviderskaya, EV; Bennett, DC; Raposo-Benedetti, G; Owen, DJ; Dennis, MK; Marks, MS (2021) A BLOC-1-AP-3 super-complex sorts a cis-SNARE complex into endosome-derived tubular transport carriers. J Cell Biol, 220 (7). e202005173. ISSN 1540-8140 https://doi.org/10.1083/jcb.202005173
SGUL Authors: Sviderskaya, Elena Vladimirovna

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Abstract

Membrane transport carriers fuse with target membranes through engagement of cognate vSNAREs and tSNAREs on each membrane. How vSNAREs are sorted into transport carriers is incompletely understood. Here we show that VAMP7, the vSNARE for fusing endosome-derived tubular transport carriers with maturing melanosomes in melanocytes, is sorted into transport carriers in complex with the tSNARE component STX13. Sorting requires either recognition of VAMP7 by the AP-3δ subunit of AP-3 or of STX13 by the pallidin subunit of BLOC-1, but not both. Consequently, melanocytes expressing both AP-3δ and pallidin variants that cannot bind their respective SNARE proteins are hypopigmented and fail to sort BLOC-1-dependent cargo, STX13, or VAMP7 into transport carriers. However, SNARE binding does not influence BLOC-1 function in generating tubular transport carriers. These data reveal a novel mechanism of vSNARE sorting by recognition of redundant sorting determinants on a SNARE complex by an AP-3-BLOC-1 super-complex.

Item Type: Article
Additional Information: © 2021 Bowman et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
Keywords: 06 Biological Sciences, 11 Medical and Health Sciences, Developmental Biology
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Journal or Publication Title: J Cell Biol
ISSN: 1540-8140
Language: eng
Dates:
DateEvent
5 July 2021Published
22 April 2021Published Online
19 March 2021Accepted
Publisher License: Publisher's own licence
Projects:
Project IDFunderFunder ID
F32 AR062476NIAMS NIH HHSUNSPECIFIED
T32 GM007229NIGMS NIH HHSUNSPECIFIED
R01 EY015625NEI NIH HHSUNSPECIFIED
K12 GM081259NIGMS NIH HHSUNSPECIFIED
108429/Z/15/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
R15 GM132810NIGMS NIH HHSUNSPECIFIED
207455/Z/17/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
FSU-201811782MJ Murdock Charitable Trusthttp://dx.doi.org/10.13039/100000937
PubMed ID: 33886957
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/113242
Publisher's version: https://doi.org/10.1083/jcb.202005173

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