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Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins.

Alzahofi, N; Welz, T; Robinson, CL; Page, EL; Briggs, DA; Stainthorp, AK; Reekes, J; Elbe, DA; Straub, F; Kallemeijn, WW; et al. Alzahofi, N; Welz, T; Robinson, CL; Page, EL; Briggs, DA; Stainthorp, AK; Reekes, J; Elbe, DA; Straub, F; Kallemeijn, WW; Tate, EW; Goff, PS; Sviderskaya, EV; Cantero, M; Montoliu, L; Nedelec, F; Miles, AK; Bailly, M; Kerkhoff, E; Hume, AN (2020) Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins. Nat Commun, 11 (1). p. 3495. ISSN 2041-1723 https://doi.org/10.1038/s41467-020-17212-6
SGUL Authors: Sviderskaya, Elena Vladimirovna

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Abstract

Cell biologists generally consider that microtubules and actin play complementary roles in long- and short-distance transport in animal cells. On the contrary, using melanosomes of melanocytes as a model, we recently discovered that the motor protein myosin-Va works with dynamic actin tracks to drive long-range organelle dispersion in opposition to microtubules. This suggests that in animals, as in yeast and plants, myosin/actin can drive long-range transport. Here, we show that the SPIRE-type actin nucleators (predominantly SPIRE1) are Rab27a effectors that co-operate with formin-1 to generate actin tracks required for myosin-Va-dependent transport in melanocytes. Thus, in addition to melanophilin/myosin-Va, Rab27a can recruit SPIREs to melanosomes, thereby integrating motor and track assembly activity at the organelle membrane. Based on this, we suggest a model in which organelles and force generators (motors and track assemblers) are linked, forming an organelle-based, cell-wide network that allows their collective activity to rapidly disperse the population of organelles long-distance throughout the cytoplasm.

Item Type: Article
Additional Information: For Supplementary Movie 1, 2 and 3, please see publisher website. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Keywords: MD Multidisciplinary
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Journal or Publication Title: Nat Commun
ISSN: 2041-1723
Language: eng
Dates:
DateEvent
13 July 2020Published
4 June 2020Accepted
Publisher License: Creative Commons: Attribution 4.0
Projects:
Project IDFunderFunder ID
108429/Z/15/ZWellcome Trust (Wellcome)http://dx.doi.org/10.13039/100010269
204843/Z/16/ZWellcome Trust (Wellcome)http://dx.doi.org/10.13039/100010269
SPP1464Deutsche Forschungsgemeinschaft (German Research Foundation)UNSPECIFIED
KE 447/10-2Deutsche Forschungsgemeinschaft (German Research Foundation)UNSPECIFIED
KE 447/18-1Deutsche Forschungsgemeinschaft (German Research Foundation)UNSPECIFIED
G1100063Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
BB/F016956/1RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)UNSPECIFIED
PubMed ID: 32661310
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/112172
Publisher's version: https://doi.org/10.1038/s41467-020-17212-6

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