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BLOC-1 and BLOC-3 regulate VAMP7 cycling to and from melanosomes via distinct tubular transport carriers.

Dennis, MK; Delevoye, C; Acosta-Ruiz, A; Hurbain, I; Romao, M; Hesketh, GG; Goff, PS; Sviderskaya, EV; Bennett, DC; Luzio, JP; et al. Dennis, MK; Delevoye, C; Acosta-Ruiz, A; Hurbain, I; Romao, M; Hesketh, GG; Goff, PS; Sviderskaya, EV; Bennett, DC; Luzio, JP; Galli, T; Owen, DJ; Raposo, G; Marks, MS (2016) BLOC-1 and BLOC-3 regulate VAMP7 cycling to and from melanosomes via distinct tubular transport carriers. The Journal of Cell Biology (JCB), 214 (3). pp. 293-308. ISSN 1540-8140 https://doi.org/10.1083/jcb.201605090
SGUL Authors: Bennett, Dorothy Catherine Sviderskaya, Elena Vladimirovna

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Abstract

Endomembrane organelle maturation requires cargo delivery via fusion with membrane transport intermediates and recycling of fusion factors to their sites of origin. Melanosomes and other lysosome-related organelles obtain cargoes from early endosomes, but the fusion machinery involved and its recycling pathway are unknown. Here, we show that the v-SNARE VAMP7 mediates fusion of melanosomes with tubular transport carriers that also carry the cargo protein TYRP1 and that require BLOC-1 for their formation. Using live-cell imaging, we identify a pathway for VAMP7 recycling from melanosomes that employs distinct tubular carriers. The recycling carriers also harbor the VAMP7-binding scaffold protein VARP and the tissue-restricted Rab GTPase RAB38. Recycling carrier formation is dependent on the RAB38 exchange factor BLOC-3. Our data suggest that VAMP7 mediates fusion of BLOC-1-dependent transport carriers with melanosomes, illuminate SNARE recycling from melanosomes as a critical BLOC-3-dependent step, and likely explain the distinct hypopigmentation phenotypes associated with BLOC-1 and BLOC-3 deficiency in Hermansky-Pudlak syndrome variants.

Item Type: Article
Additional Information: © 2016 Dennis et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
Keywords: Developmental Biology, 06 Biological Sciences, 11 Medical And Health Sciences
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Cell Sciences (INCCCS)
Journal or Publication Title: The Journal of Cell Biology (JCB)
ISSN: 1540-8140
Language: ENG
Dates:
DateEvent
1 August 2016Published
5 July 2016Accepted
Publisher License: Creative Commons: Attribution-Noncommercial-Share Alike 3.0 IGO
Projects:
Project IDFunderFunder ID
R01 GM108807National Institute of General Medical Scienceshttp://dx.doi.org/10.13039/100000057
F32 AR062476National Institute of Arthritis and Musculoskeletal and Skin Diseaseshttp://dx.doi.org/10.13039/100000069
R01 EY015625National Eye Institutehttp://dx.doi.org/10.13039/100000053
R01 AR048155National Institute of Arthritis and Musculoskeletal and Skin Diseaseshttp://dx.doi.org/10.13039/100000069
R01 HL121323National Heart, Lung, and Blood Institutehttp://dx.doi.org/10.13039/100000050
PubMed ID: 27482051
Web of Science ID: WOS:000380831800009
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/108097
Publisher's version: https://doi.org/10.1083/jcb.201605090

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