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Dentate gyrus progenitor cell proliferation after the onset of spontaneous seizures in the tetanus toxin model of temporal lobe epilepsy.

Jiruska, P; Shtaya, ABY; Bodansky, DMS; Chang, W-C; Gray, WP; Jefferys, JGR (2013) Dentate gyrus progenitor cell proliferation after the onset of spontaneous seizures in the tetanus toxin model of temporal lobe epilepsy. Neurobiol Dis, 54. pp. 492-498. ISSN 1095-953X https://doi.org/10.1016/j.nbd.2013.02.001
SGUL Authors: Shtaya, Anan BY

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Abstract

Temporal lobe epilepsy alters adult neurogenesis. Existing experimental evidence is mainly from chronic models induced by an initial prolonged status epilepticus associated with substantial cell death. In these models, neurogenesis increases after status epilepticus. To test whether status epilepticus is necessary for this increase, we examined precursor cell proliferation and neurogenesis after the onset of spontaneous seizures in a model of temporal lobe epilepsy induced by unilateral intrahippocampal injection of tetanus toxin, which does not cause status or, in most cases, detectable neuronal loss. We found a 4.5 times increase in BrdU labeling (estimating precursor cells proliferating during the 2nd week after injection of toxin and surviving at least up to 7days) in dentate gyri of both injected and contralateral hippocampi of epileptic rats. Radiotelemetry revealed that the rats experienced 112±24 seizures, lasting 88±11s each, over a period of 8.6±1.3days from the first electrographic seizure. On the first day of seizures, their duration was a median of 103s, and the median interictal period was 23min, confirming the absence of experimentally defined status epilepticus. The total increase in cell proliferation/survival was due to significant population expansions of: radial glial-like precursor cells (type I; 7.2×), non-radial type II/III neural precursors in the dentate gyrus stem cell niche (5.6×), and doublecortin-expressing neuroblasts (5.1×). We conclude that repeated spontaneous brief temporal lobe seizures are sufficient to promote increased hippocampal neurogenesis in the absence of status epilepticus.

Item Type: Article
Additional Information: © 2013 Elsevier Inc. Open access under CC BY license (http://creativecommons.org/licenses/by/3.0/).
Keywords: Animals, Cell Proliferation, Dentate Gyrus, Disease Models, Animal, Electrophysiology, Epilepsy, Temporal Lobe, Immunohistochemistry, Male, Neural Stem Cells, Neurogenesis, Neurotoxins, Rats, Rats, Sprague-Dawley, Seizures, Tetanus Toxin, Dentate Gyrus, Animals, Rats, Rats, Sprague-Dawley, Epilepsy, Temporal Lobe, Seizures, Disease Models, Animal, Tetanus Toxin, Neurotoxins, Immunohistochemistry, Electrophysiology, Cell Proliferation, Male, Neurogenesis, Neural Stem Cells, Neurology & Neurosurgery, 1103 Clinical Sciences, 1109 Neurosciences
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Neuroscience (INCCNS)
Journal or Publication Title: Neurobiol Dis
ISSN: 1095-953X
Language: eng
Dates:
DateEvent
June 2013Published
21 February 2013Published Online
6 February 2013Accepted
Publisher License: Creative Commons: Attribution 3.0
Projects:
Project IDFunderFunder ID
G0802162Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
G0300356Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
G0801418Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
G0802162Minnesota Department of Healthhttp://dx.doi.org/10.13039/100004956
PubMed ID: 23439313
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/107628
Publisher's version: https://doi.org/10.1016/j.nbd.2013.02.001

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