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Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis.

Perin, L; Sedrakyan, S; Giuliani, S; Da Sacco, S; Carraro, G; Shiri, L; Lemley, KV; Rosol, M; Wu, S; Atala, A; et al. Perin, L; Sedrakyan, S; Giuliani, S; Da Sacco, S; Carraro, G; Shiri, L; Lemley, KV; Rosol, M; Wu, S; Atala, A; Warburton, D; De Filippo, RE (2010) Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis. PLoS One, 5 (2). ISSN 1932-6203 https://doi.org/10.1371/journal.pone.0009357
SGUL Authors: Giuliani, Stefano

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Abstract

Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kit(pos) stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system. Herein, we show for the first time the successful therapeutic application of hAFSC in a mouse model with glycerol-induced rhabdomyolysis and ATN. When injected into the damaged kidney, luciferase-labeled hAFSC can be tracked using bioluminescence. Moreover, we show that hAFSC provide a protective effect, ameliorating ATN in the acute injury phase as reflected by decreased creatinine and BUN blood levels and by a decrease in the number of damaged tubules and apoptosis therein, as well as by promoting proliferation of tubular epithelial cells. We show significant immunomodulatory effects of hAFSC, over the course of ATN. We therefore speculate that AFSC could represent a novel source of stem cells that may function to modulate the kidney immune milieu in renal failure caused by ATN.

Item Type: Article
Additional Information: ©2010 Perin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Amniotic Fluid, Animals, Apoptosis, Blood Urea Nitrogen, Cell Proliferation, Creatinine, Cytokines, Disease Models, Animal, Embryonic Stem Cells, Gene Expression, Glycerol, Humans, Karyotyping, Kidney, Kidney Tubular Necrosis, Acute, Luciferases, Luminescent Measurements, Membrane Proteins, Mice, Mice, Nude, PAX2 Transcription Factor, Reverse Transcriptase Polymerase Chain Reaction, Rhabdomyolysis, Stem Cell Transplantation, Transplantation, Heterologous, General Science & Technology, MD Multidisciplinary
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: PLoS One
Article Number: e9357
ISSN: 1932-6203
Language: eng
Dates:
DateEvent
24 February 2010Published
PubMed ID: 20195358
Web of Science ID: WOS:000274924200010
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/107289
Publisher's version: https://doi.org/10.1371/journal.pone.0009357

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