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Proteomic analysis of the Plasmodium male gamete reveals the key role for glycolysis in flagellar motility.

Talman, AM; Prieto, JH; Marques, S; Ubaida-Mohien, C; Lawniczak, M; Wass, MN; Xu, T; Frank, R; Ecker, A; Stanway, RS; et al. Talman, AM; Prieto, JH; Marques, S; Ubaida-Mohien, C; Lawniczak, M; Wass, MN; Xu, T; Frank, R; Ecker, A; Stanway, RS; Krishna, S; Sternberg, MJ; Christophides, GK; Graham, DR; Dinglasan, RR; Yates, JR; Sinden, RE (2014) Proteomic analysis of the Plasmodium male gamete reveals the key role for glycolysis in flagellar motility. Malaria Journal, 13 (315). ISSN 1475-2875 https://doi.org/10.1186/1475-2875-13-315
SGUL Authors: Krishna, Sanjeev

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Abstract

BACKGROUND: Gametogenesis and fertilization play crucial roles in malaria transmission. While male gametes are thought to be amongst the simplest eukaryotic cells and are proven targets of transmission blocking immunity, little is known about their molecular organization. For example, the pathway of energy metabolism that power motility, a feature that facilitates gamete encounter and fertilization, is unknown. METHODS: Plasmodium berghei microgametes were purified and analysed by whole-cell proteomic analysis for the first time. Data are available via ProteomeXchange with identifier PXD001163. RESULTS: 615 proteins were recovered, they included all male gamete proteins described thus far. Amongst them were the 11 enzymes of the glycolytic pathway. The hexose transporter was localized to the gamete plasma membrane and it was shown that microgamete motility can be suppressed effectively by inhibitors of this transporter and of the glycolytic pathway. CONCLUSIONS: This study describes the first whole-cell proteomic analysis of the malaria male gamete. It identifies glycolysis as the likely exclusive source of energy for flagellar beat, and provides new insights in original features of Plasmodium flagellar organization.

Item Type: Article
Additional Information: © 2014 Talman et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Keywords: Gamete, Plasmodium, Glycolysis, Flagellum, Energy metabolism, Science & Technology, Life Sciences & Biomedicine, Infectious Diseases, Parasitology, Tropical Medicine, PARASITOLOGY, MEMBRANE-PROTEIN TOPOLOGY, TANDEM MASS-SPECTRA, MALARIA PARASITE, SUBCELLULAR-LOCALIZATION, HAEMOPROTEUS-COLUMBAE, CHLAMYDOMONAS FLAGELLA, YOELII-NIGERIENSIS, HEXOSE TRANSPORTER, MICROTUBULE MOTOR, LIFE-CYCLE, 1108 Medical Microbiology
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: Malaria Journal
ISSN: 1475-2875
Language: eng
Dates:
DateEvent
13 August 2014Published
PubMed ID: 25124718
Web of Science ID: WOS:000341033500001
Go to PubMed abstract
URI: https://openaccess.sgul.ac.uk/id/eprint/107272
Publisher's version: https://doi.org/10.1186/1475-2875-13-315

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