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Comparison of effects of green tea catechins on apicomplexan hexose transporters and mammalian orthologues

Slavic, K; Derbyshire, ET; Naftalin, RJ; Krishna, S; Staines, HM (2009) Comparison of effects of green tea catechins on apicomplexan hexose transporters and mammalian orthologues. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 168 (1). 113 - 116. ISSN 0166-6851 https://doi.org/10.1016/j.molbiopara.2009.06.008
SGUL Authors: Krishna, Sanjeev Staines, Henry Michael Slavic, Ksenija

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Abstract

Here we have investigated the inhibitory properties of green tea catechins on the Plasmodium falciparum hexose transporter (PfHT), the Babesia bovis hexose transporter 1 (BboHT1) and the mammalian facilitative glucose transporters, GLUT1 and GLUT5, expressed in Xenopus laevis oocytes. (−)-Epicatechin-gallate (ECG) and (−)-epigallocatechin-gallate (EGCG) inhibited d-glucose transport by GLUT1 and PfHT, and d-fructose transport by GLUT5, with apparent Ki values between 45 and 117 μM. BboHT1 was more potently inhibited by the ungallated catechins (−)-epicatechin (EC) and (−)-epigallocatechin (EGC), with apparent Ki values of 108 and 168 μM, respectively. Site-directed mutagenesis experiments provided little further support for previously reported models of catechin binding to hexose transporters. Furthermore, P. falciparum growth inhibition by catechins was not affected by the external d-glucose concentration. Our results provide new data on the inhibitory action of catechins against sugar transporters but were unable to elucidate the antimalarial mechanism of action of these agents.

Item Type: Article
Additional Information: © 2009 Elsevier B.V. Under a Creative Commons license (CC BY 3.0).
Keywords: Animals, Babesia bovis, Catechin, Enzyme Inhibitors, Glucose Transporter Type 1, Glucose Transporter Type 5, Mammals, Monosaccharide Transport Proteins, Oocytes, Plasmodium falciparum, Tea, Xenopus laevis, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Parasitology, Glucose, Transport, GLUT1/5, PfHT, BboHT1, Oocyte, PLASMODIUM-FALCIPARUM, GLUCOSE-TRANSPORTER, BINDING-SITE, GALLATE, GLUT1, FABG
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: MOLECULAR AND BIOCHEMICAL PARASITOLOGY
ISSN: 0166-6851
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Dates:
DateEvent
1 November 2009Published
Web of Science ID: WOS:000270019800015
Download EPMC Full text (HTML)
URI: http://openaccess.sgul.ac.uk/id/eprint/96
Publisher's version: https://doi.org/10.1016/j.molbiopara.2009.06.008

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