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Extracellular matrix composition and remodeling in human abdominal aortic aneurysms: a proteomics approach.

Didangelos, A; Yin, X; Mandal, K; Saje, A; Smith, A; Xu, Q; Jahangiri, M; Mayr, M (2011) Extracellular matrix composition and remodeling in human abdominal aortic aneurysms: a proteomics approach. Molecular and Cellular Proteomics, 10 (8). ISSN 1535-9484 https://doi.org/10.1074/mcp.M111.008128
SGUL Authors: Jahangiri, Marjan

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Abstract

Abdominal aortic aneurysms (AAA) are characterized by pathological remodeling of the aortic extracellular matrix (ECM). However, besides the well-characterized elastolysis and collagenolysis little is known about changes in other ECM proteins. Previous proteomics studies on AAA focused on cellular changes without emphasis on the ECM. In the present study, ECM proteins and their degradation products were selectively extracted from aneurysmal and control aortas using a solubility-based subfractionation methodology and analyzed by gel-liquid chromatography-tandem MS and label-free quantitation. The proteomics analysis revealed novel changes in the ECM of AAA, including increased expression as well as degradation of collagen XII, thrombospondin 2, aortic carboxypeptidase-like protein, periostin, fibronectin and tenascin. Proteomics also confirmed the accumulation of macrophage metalloelastase (MMP-12). Incubation of control aortic tissue with recombinant MMP-12 resulted in the extensive fragmentation of these glycoproteins, most of which are novel substrates of MMP-12. In conclusion, our proteomics methodology allowed the first detailed analysis of the ECM in AAA and identified markers of pathological ECM remodeling related to MMP-12 activity.

Item Type: Article
Additional Information: © 2011 by The American Society for Biochemistry and Molecular Biology, Inc. Creative Commons Attribution Non-Commercial License applies to Author Choice Articles.
Keywords: Adult, Aged, Aorta, Abdominal, Aortic Aneurysm, Abdominal, Carboxypeptidases, Case-Control Studies, Chemical Fractionation, Cluster Analysis, Extracellular Matrix Proteins, Female, Glycoproteins, Guanidine, Humans, Inflammation Mediators, Male, Matrix Metalloproteinase 12, Middle Aged, Proteome, Proteomics, Repressor Proteins, Solubility, Young Adult, Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Biochemistry & Molecular Biology, BIOCHEMICAL RESEARCH METHODS, CARBOXYPEPTIDASE-LIKE PROTEIN, SHOTGUN PROTEOMICS, STATISTICAL-MODEL, OXIDATIVE STRESS, GELATINASE-B, EXPRESSION, METALLOPROTEINASES, INFLAMMATION, CARTILAGE, MICE, Biochemistry & Molecular Biology, MD Multidisciplinary
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Cardiac (INCCCA)
Journal or Publication Title: Molecular and Cellular Proteomics
Article Number: M111.008128
ISSN: 1535-9484
Language: eng
Dates:
DateEvent
August 2011Published
Projects:
Project IDFunderFunder ID
FS/08/002/24537British Heart FoundationUNSPECIFIED
UNSPECIFIEDBritish Heart FoundationUNSPECIFIED
PubMed ID: 21593211
Web of Science ID: WOS:000293386400012
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URI: http://openaccess.sgul.ac.uk/id/eprint/881
Publisher's version: https://doi.org/10.1074/mcp.M111.008128

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