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Decreased MCM2-6 in Drosophila S2 cells does not generate significant DNA damage or cause a marked increase in sensitivity to replication interference.

Crevel, I; Crevel, G; Gostan, T; de Renty, C; Coulon, V; Cotterill, S (2011) Decreased MCM2-6 in Drosophila S2 cells does not generate significant DNA damage or cause a marked increase in sensitivity to replication interference. PLOS ONE, 6 (11). e27101 -. ISSN 1932-6203 https://doi.org/10.1371/journal.pone.0027101
SGUL Authors: Cotterill, Susan Margaret

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Abstract

A reduction in the level of some MCM proteins in human cancer cells (MCM5 in U20S cells or MCM3 in Hela cells) causes a rapid increase in the level of DNA damage under normal conditions of cell proliferation and a loss of viability when the cells are subjected to replication interference. Here we show that Drosophila S2 cells do not appear to show the same degree of sensitivity to MCM2-6 reduction. Under normal cell growth conditions a reduction of >95% in the levels of MCM3, 5, and 6 causes no significant short term alteration in the parameters of DNA replication or increase in DNA damage. MCM depleted cells challenged with HU do show a decrease in the density of replication forks compared to cells with normal levels of MCM proteins, but this produces no consistent change in the levels of DNA damage observed. In contrast a comparable reduction of MCM7 levels has marked effects on viability, replication parameters and DNA damage in the absence of HU treatment.

Item Type: Article
Additional Information: ©2011 Crevel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Animals, Blotting, Western, Cell Cycle Proteins, Cell Proliferation, Cells, Cultured, Chromosomal Proteins, Non-Histone, DNA Damage, DNA Replication, Drosophila, Drosophila Proteins, Flow Cytometry, Humans, Hydroxyurea, Nucleic Acid Synthesis Inhibitors
SGUL Research Institute / Research Centre: Academic Structure > Molecular and Clinical Sciences Research Institute (MCS)
Academic Structure > Molecular and Clinical Sciences Research Institute (MCS) > Cell Sciences (INCCCS)
Journal or Publication Title: PLOS ONE
ISSN: 1932-6203
Dates:
DateEvent
15 November 2011Published
PubMed ID: 22102875
Web of Science ID: 22102875
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URI: http://openaccess.sgul.ac.uk/id/eprint/2585
Publisher's version: https://doi.org/10.1371/journal.pone.0027101

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