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Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelope protein CN54gp140 within novel rheologically structured vehicles elicits specific immune responses

Curran, RM; Donnelly, L; Morrow, RJ; Fraser, C; Andrews, G; Cranage, M; Malcolm, RK; Shattock, RJ; Woolfson, AD (2009) Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelope protein CN54gp140 within novel rheologically structured vehicles elicits specific immune responses. VACCINE, 27 (48). 6791 - 6798. ISSN 0264-410X https://doi.org/10.1016/j.vaccine.2009.08.088
SGUL Authors: Cranage, Martin Patrick Shattock, Robin John

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Abstract

Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrix-forming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.

Item Type: Article
Additional Information: © 2009 Elsevier Ltd. Under a Creative Commons license CC BY 3.0
Keywords: AIDS Vaccines, Administration, Intravaginal, Animals, Antibody Formation, Enzyme-Linked Immunosorbent Assay, Female, Gels, HIV Antibodies, Immunity, Mucosal, Immunoglobulin A, Secretory, Immunoglobulin G, Rabbits, Rheology, env Gene Products, Human Immunodeficiency Virus, Science & Technology, Life Sciences & Biomedicine, Immunology, Medicine, Research & Experimental, Research & Experimental Medicine, Vaginal delivery, Mucosal vaccination, HIV-1, GENITAL-TRACT, IN-VITRO, INTRAVAGINAL IMMUNIZATION, ANTIBODY-RESPONSE, ENHANCED MUCOSAL, VACCINE, MUCOADHESIVE, SYSTEMS, INFECTION, HIV/AIDS
SGUL Research Institute / Research Centre: Academic Structure > Infection and Immunity Research Institute (INII)
Journal or Publication Title: VACCINE
ISSN: 0264-410X
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Dates:
DateEvent
12 November 2009Published
Web of Science ID: WOS:000272088700020
Download EPMC Full text (HTML)
URI: http://openaccess.sgul.ac.uk/id/eprint/1375
Publisher's version: https://doi.org/10.1016/j.vaccine.2009.08.088

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